FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331478
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32430.24356391919355785No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31970.24010911182911018No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT31550.23695472249635366No Hit
GTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC29600.22230934345141262No Hit
GTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGTCA25570.1920422267585345No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24700.1855081345692531No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23970.18002550549089058No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23760.17844831082451232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22650.17011171044508433No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22160.16643158955686838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22010.16530502193802676No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21400.1607236469547375No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21320.16012281089135533No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCA20900.15696842155859878No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20740.1557667494318344No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG20730.1556916449239116No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGAGAGTTCCTTCTTGGACGTGTCCACGGC20380.1530629871466145No Hit
CTACAAGCCGTCCCTCCAGAGTCGAGTCACCATGGCCGTGGACACGTCCAAGAAGGAACTCTCCCTGAAGTTGAA20270.152236837559464No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19980.15005880682970352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19870.14923265724255302No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC19600.14720483552863808No Hit
CTATAATACATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGAATCCAGACCCAGTAATAACTGTTC19550.14682931298902424No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG19530.14667910397317868No Hit
ATGTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGT19380.14555253635433707No Hit
GTGTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGC19370.1454774318464143No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19220.14435086422757268No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18870.14172220645027556No Hit
GTCTGGGACCCCAGAGTCCCGGTGAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA18690.14037032530766563No Hit
GTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT18620.1398445937522062No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG18380.1380420855620596No Hit
CTAGTACACTGAACGCCGGAAAAACTCGCACAATAATACACAGCCGTGTC18050.13556363680060804No Hit
CACTTGTACTGTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGG17830.133911337626307No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA17800.1336860241025387No Hit
GGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGA17740.13323539705500204No Hit
GTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCATA17590.1321088294361604No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17080.1282784995320989No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16650.1250490056914196No Hit
GTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGCCAGGGAATCCTGGTCATCGTCTCCTCAGCCTC16620.1248236921676513No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16400.12317139299335025No Hit
GTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGGTCTGGATTCG16340.1227207659458136No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16290.12234524340619973No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16230.12189461635866308No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15610.11723813686745105No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15470.1161866737565322No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15410.11573604670899558No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15370.11543562867730446No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15350.11528541966145892No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGA15320.11506010613769059No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15000.11265676188416181No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14990.11258165737623904No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14890.1118306122970113No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA14720.11055383566232413No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA14670.11017831312271026No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14600.10965258156725084No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGT14380.10800028239294979No Hit
GTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCT14320.10754965534541315No Hit
GTTCTAGTACACTGAACGCCGGAAAAACTCGCACAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAA14120.10604756518695765No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14060.105596938139421No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13990.10507120658396157No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13920.10454547502850216No Hit
GATGGGTCCTGTCCCAATTGCAGCTGCGGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACTCTGTCCCTCA13810.10371932544135164No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13730.10311848937796944No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA13600.10214213077497337No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT13490.10131598118782285No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG13450.10101556315613175No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13410.10071514512444066No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13400.10064004061651789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3000.048.2043952
GTGGTAT3100.046.661681
GGTATCA9400.045.43251
GTATCAA25500.042.679531
TATCAAC27900.038.75112
ATCAACG29100.037.153123
TCAACGC29450.036.5946544
CAACGCA29550.036.470815
ACGGGAC3500.035.4301116
AACGCAG30400.035.3378076
CGCAGAG31850.033.74558
CAGAGTA32100.033.5912610
AGAGTAC32300.033.38326311
GCAGAGT33550.032.2421579
ACGCAGA36050.029.8106257
TATTAGA1302.0190782E-1029.1345232
AGTACGG31500.029.0909613
GAGTACG31700.029.01609412
AATGGTA2552.8376235E-1028.29775470
TTCGGGG14900.028.17689970
Produced by FastQC (version 0.11.5)