FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427793
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA43630.3055765086395577No Hit
ACCTACTACAGCCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAAA29930.20962422423978824No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29330.20542193441206114No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28290.1981379653773341No Hit
CTCTAAACTAGACGCCTTATAGATCAGGACCTTGGGGGCTTTCCCTGGCT28240.19778777455835686No Hit
GTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGGGCTGGATCCG26550.18595132487692545No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGCGACAGAATTCACTCTCACCATCAGCAGC25210.17656621092833485No Hit
GTGTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGA25090.17572575296278942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23280.1630488453158126No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23110.1618581965312899No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21780.15254312074649476No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGA21670.1517727009447448No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21150.14813071642738126No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20210.14154712903060876No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20040.1403564802460861No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20020.14021640391849519No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCACCAGTGATGATTACTACTGGG19170.13426315999588176No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18870.1321620150820182No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC18160.1271893054525411No Hit
GTAGTAGGTACTCCCACTATAATAGATACTCCCAATCCACTCCAACCCCTTCCCTGGGGGCTGGCGGATCCAGCC17890.12529827503006388No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17730.12417766440933665No Hit
GTATTACTGTGCGAGACGCCGATATAACTGGAACGAGGGGTGGTTCGACCCCTGGGGCCAGGGAGCCCTGGTCAC16360.11458243596935971No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16330.11437232147797334No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16250.11381201616760972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16030.11227117656410979No Hit
GATCAGGACCTTGGGGGCTTTCCCTGGCTTCTGCTGATACCAGGCCAACC15970.11185094758133707No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15580.10911945919331444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15580.10911945919331444No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15410.10792881040879174No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA15240.10673816162426907No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15150.10610781815011No Hit
GTCCTGATCTATAAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTT14830.10386659690865552No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14630.10246583363274649No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14630.10246583363274649No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT14580.10211564281376923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA200.007130849652.1645354
TACGGAT13450.048.69782670
GTGGTAT3300.042.82411
GTATCAA22200.038.8155171
ATCAACG26450.032.051753
CTACGGA13850.031.55562469
TCAACGC27000.031.3988424
TATCAAC27500.031.0785872
CAACGCA27550.031.0221845
TATTCGA450.00462649730.66200827
AACGCAG28150.030.240666
CGCAGAG30050.028.3474678
AGAGTAC29600.027.84730711
CAGAGTA30500.027.1386610
GCAGAGT31600.026.957959
ACGCAGA33950.025.3886897
GCGTAGA15700.025.28414570
TGTATAC1001.57736E-524.1234993
TCTACGG16250.024.10197668
CAGTTCG12100.024.05824770
Produced by FastQC (version 0.11.5)