FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514651
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60960.24241932578318026No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50850.20221493956815478No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG46040.18308703672994783No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA45560.18117822314110388No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40910.16268659149917822No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC39840.15843152787404693No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39250.1560852778377596No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38060.15135301081541733No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34290.1363608707530389No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32160.12789051045254393No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31170.1239535824255533No Hit
GTAATAATCACCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGC30380.1208119933939143No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29520.11739203571390225No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29410.11695459926645885No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG28950.11512531957715007No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC28230.11226209919388416No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG26520.10546195078362762No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCGAGGACGGCGACCTTGGTC25970.10327476854641061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58950.041.3158041
GGTATCA23100.040.7128261
TATCAAC65500.037.1082
TCGTATG1250.035.9970340
TCAACGC67650.035.572434
ATCAACG68350.035.4599653
CAACGCA68750.034.8530465
AACGCAG69850.034.402756
CGCAGAG77200.031.0387868
AGAGTAC79850.030.35483211
CAGAGTA80800.029.91271610
ACGCAGA81300.029.599947
GTGGTAT9000.029.4733031
GCAGAGT83550.028.720979
TGGTATC8900.028.6250272
CGTTACG500.00781657827.54191417
AGTACGG56800.026.24639513
TCTCGTA2000.025.93192138
GAGTACG57550.025.90434612
CCCACGA4900.024.59148419
Produced by FastQC (version 0.11.5)