FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2514651
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52790.20992972782306574No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC48520.19294924027230814No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT47080.18722279950577636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45730.18185426128715276No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45140.1795080112508654No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA39730.15799409142660353No Hit
GCCTTGGGCTGACCGAGGACGGCGACCTTGGTCCCAGTTCCGAAGACGTGACCATTCAGGCTGTCATCCCATG37710.14996116757355196No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT36660.14578563784795584No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC36460.1449902988526042No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC34630.13771294704513667No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33800.13441229021442735No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC32270.12832794689998733No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30880.12280034088229341No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29530.11743180266366982No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC28700.11413114583296052No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCACGTCTTCGG27770.11043281950457537No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25790.10255896345059415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55000.040.7036931
GGTATCA21950.040.1070751
TATCAAC61350.036.3152962
TCAACGC62450.035.565524
ATCAACG63550.034.9499133
CAACGCA64200.034.703175
AACGCAG65900.034.0213666
TGGTATC9200.030.6446862
CGCAGAG73500.030.4694528
AGAGTAC74500.030.01548411
CAGAGTA75050.029.84076310
GTGGTAT10150.029.8148171
ACGCAGA77050.029.1085157
GCAGAGT79300.028.3276799
AGTACGG54750.025.32265313
CGTCTAA2350.025.18596350
GAGTACG55450.025.00298112
GCGTAGA902.177335E-422.9212282
CTCGTAT3100.022.63826460
GTACTTT37650.022.2952914
Produced by FastQC (version 0.11.5)