FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196218
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG26030.21760247714045436No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA22160.18525051453831992No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21710.18148865842179268No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19840.1658560563375572No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17990.1503906478585007No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17510.14637800133420498No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17330.1448732588875941No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGA17160.14345211324357265No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16760.14010824113999287No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16230.1356776106027497No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16050.13417286815613877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15870.13266812570952785No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15080.1260639783049578No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14330.1197942181107457No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13520.11302287710099665No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13290.11110015064143826No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACTCCAGTAGTAATCACCA13160.11001339220777484No Hit
GTATGATAATCTCCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13120.10967900499741685No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12370.10340924480320476No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12360.10332564800061526No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12170.10173730875141487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20200.035.3414571
GTGGTAT3250.034.6832771
TCAACGC22750.031.2090784
ATCAACG22800.031.1406353
TACGCAT553.4455443E-431.06421328
TACTGAT12100.030.7502270
TATCAAC23300.030.6188832
CAACGCA23450.030.4230275
AACGCAG24300.029.499326
AGAGTAC25950.027.88672411
CAGAGTA26400.027.5406810
CGCAGAG26900.026.774988
GCAGAGT27350.026.334449
ACGGGTA801.0279151E-425.60119616
TATAGGG801.0279151E-425.6011962
GCTACGT951.1411794E-525.1520523
CCTATGC1101.2648052E-624.8254013
ACGCAGA29600.024.3326667
ATATAGC851.5443408E-424.1113681
TTGACCG750.00212022222.75661812
Produced by FastQC (version 0.11.5)