FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1196218
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGAGTTGGATCCG23940.20013074539924997No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGCTCAGCTTCAGGGAGA23140.1934430011920904No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22450.18767482181341527No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT19580.16368253947023034No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17790.1487187118067108No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17130.14320132283580417No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGATTACTACTGGA17070.1426997420202672No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16350.1366807722338236No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15450.12915706000076907No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14870.12430844545057841No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14590.12196773497807256No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14350.11996141171592468No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13170.11009698901036434No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12760.10666952010419506No Hit
GTGTATTACTGTGCCAGGACGACCGACATCTATGGCTCCACCCTCGGCCT12600.10533197126276314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12390.10357643840838376No Hit
GTAATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACTCCAGTAGTAATCACCA12360.10332564800061526No Hit
GTATGATAATCTCCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT12260.10248967997472033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3450.045.468561
TATATCG306.695881E-445.4514585
GTATCAA21300.038.4241371
TACTGAT11250.037.61093570
TAACATA800.002739067434.12282670
TATCAAC24700.032.984512
ATCAACG25200.032.0595133
TCAACGC25350.031.8698084
CAACGCA26000.031.2041765
AACGCAG26400.030.8617976
AGAGTAC27550.029.34079611
CAGAGTA28400.028.58273110
CGCAGAG28850.028.2551238
ACTATCG500.00707097628.11221954
GCAGAGT29650.027.8378589
GTCTAGA756.67124E-527.281141
ACGCAGA31450.026.0266087
AGTACGG27250.025.65857313
TGTTAGA6200.025.31693570
GAGTACG28200.024.79418612
Produced by FastQC (version 0.11.5)