FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364951
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30020.21993463501620206No Hit
CTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC24000.17583048768783643No Hit
GTATGGTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGAACTGTGGCTGCACCAT23550.1725336660436895No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20130.1474778215481728No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19220.14081091555667566No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19060.1396387123054234No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18510.13560926362924383No Hit
GTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGA18280.13392422145556873No Hit
CCTTCCACCCTGTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCG17680.1295284592633728No Hit
GTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA17620.12908888304415325No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGGTAGTAAT17530.12842951871532385No Hit
GATTACTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACT17150.1256455359935998No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16810.12315460408468876No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16190.1186123164860863No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15110.11069994454013367No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15110.11069994454013367No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14990.10982079210169449No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14740.10798922452161287No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT14320.10491219098707572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)