FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364951
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30370.22249882962831632No Hit
CTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC23440.17172777630845357No Hit
GTATGGTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGAACTGTGGCTGCACCAT22760.16674591249063153No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18590.13619536525486994No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC18310.13414400956517852No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17910.13121350143704794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17190.12593858680641284No Hit
GTAGTAATCCGTACACTTTTGGCCAGGGGACCAAGCTGGACATCGAACGA16770.12286155327187569No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGGTAGTAAT16550.12124977380140387No Hit
GATTACTACCATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACT16190.1186123164860863No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16110.1180262148604602No Hit
GTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA15920.11663422349959815No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15540.11385024077787406No Hit
CCTTCCACCCTGTCTGCATCTATGGGAGACAGAGTCACCATCACTTGCCG15330.11231172401060552No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14740.10798922452161287No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14640.1072565974895802No Hit
GTGCAGCCACAGTTCGTTCGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG13890.10176189474933532No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13860.10154210663972554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)