FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492338
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAATACTTACCCCTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT54970.3683481892171881No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36780.2464589121231249No Hit
GCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGC35710.23928895464700356No Hit
GTATTATGCTGTAGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC35240.23613953407338015No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33810.22655725445576003No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33720.22595417392038533No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32430.21731001958001472No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA31040.20799577575589442No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29720.19915059457039894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28710.19238269078452735No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24260.16256370875766749No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24100.16149156558366806No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23990.16075446715154343No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23520.15760504657792002No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23380.15666692130067048No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22790.1527133933465475No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21570.14453830164480166No Hit
GTCTCTGGTGGCTCCATCAATGATTACTTCTGGAGTTGGATCCGGCAGAC21520.14420325690292682No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGG21320.1428630779354275No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20880.13991468420692899No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20630.13823946049755484No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20210.13542508466580627No Hit
CCTACAACCCGTCCCTCAACAGTCGAGTCACCATATCAGTCGACACGCTC20100.13468798623368164No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19580.1312035209181834No Hit
ATGCAGCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAACTACTAATGCC19380.12986334195068408No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19380.12986334195068408No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19370.12979633300230914No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19090.12792008244781009No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTC18890.12657990348031076No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18780.12584280504818612No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA18670.12510570661606152No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGTAGTTATTTAGCCTGGTTTCAGCAGAAA18190.12188927709406315No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18030.12081713392006369No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT17870.11974499074606423No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17620.11806976703669007No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17470.11706463281106558No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17230.1154564180500664No Hit
ATACTTACCCCTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGA16670.11170391694106832No Hit
ACCTACAACCCGTCCCTCAACAGTCGAGTCACCATATCAGTCGACACGCT16240.10882253216094478No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16110.10795141583207021No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15540.10413190577469715No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15510.10393087892957226No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15210.10192061047832328No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC14970.10031239571732409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4100.042.857662
GTATCAA24300.040.0106961
GGTATCA9850.038.502571
GTGGTAT4650.037.8138161
TATCAAC28850.034.0362052
ATCAACG29450.032.9917953
CAACGCA29800.032.6043055
TCAACGC29900.032.3800354
CGTTCCG450.003781928731.9512867
AACGCAG30650.031.7001136
TCGTATG703.7762416E-529.65737340
CGCAGAG33100.029.56298
AGAGTAC33850.028.90885711
CAGAGTA34150.028.654910
GCAGAGT35350.027.6821759
ATAATAC16550.027.4800873
TAATACT17400.027.1277314
GTGTTAC1155.6776116E-826.9822351
CATAATA16800.026.8660722
AATACTT17000.026.5500015
Produced by FastQC (version 0.11.5)