FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492338
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAATACTTACCCCTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGGCTGCACCAT52990.3550804174389448No Hit
GTATTATGCTGTAGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC41350.2770820015304844No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35070.23500038195100573No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33270.22293877124351186No Hit
GCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGC31560.21148024107139266No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31550.21141323212301769No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA30440.20397523885339647No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27730.1858158138437807No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27400.18360451854740684No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27020.18105817850915812No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26050.1745583105167864No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24440.1637698698284169No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24160.16189361927391785No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22750.15244535755304764No Hit
GTCTCTGGTGGCTCCATCAATGATTACTTCTGGAGTTGGATCCGGCAGAC22040.14768772221842505No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21290.1426620510903026No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21210.14212597950330288No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20790.1393116036715543No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20230.13555910256255618No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19250.12899222562180954No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACTTTGGTCCCTTGGCCGAACGTC18940.1269149482221856No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA18650.12497168871931158No Hit
CCCCTGGACGTTCGGCCAAGGGACCAAAGTGGAAATCAAACGAACTGTGG18330.12282740237131266No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17990.12054909812656382No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17880.11981199969443919No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17690.11853882967531484No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17630.11813677598506506No Hit
CCTACAACCCGTCCCTCAACAGTCGAGTCACCATATCAGTCGACACGCTC17460.11699762386269064No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT17220.11538940910169145No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16790.1125080243215679No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16750.11223998852806805No Hit
ATGCAGCATAGATCAGGCGCTTAGGGACTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATAACTACTAATGCC16640.11150289009594341No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16390.10982766638656927No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16160.10828646057394503No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16070.10768338003857036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15720.10533806684544654No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15720.10533806684544654No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGTAGTTATTTAGCCTGGTTTCAGCAGAAA15340.10279172680719784No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14950.10017837782057416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.040.9992871
GGTATCA11250.039.7628021
TGGATTG10150.039.4754370
GTATCAA25900.038.927281
TGGTATC4900.037.2103772
TATCAAC30300.033.3802032
ATCAACG30200.033.376823
TCAACGC30450.033.102794
CAACGCA31250.032.255365
AACGCAG31500.031.8965666
AGAGTAC32800.030.12702811
CGCAGAG33600.030.1250048
TATACTG1151.7571438E-929.9148245
CAGAGTA33850.029.4976610
GCAGAGT35800.028.179479
ATGGATT11200.027.15148469
TGTAAGG1155.799302E-826.9233422
ACGCAGA37600.026.8241567
AGTACGG33450.024.29204613
GGGAACG7450.024.28862670
Produced by FastQC (version 0.11.5)