FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108551
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10650.043.401471
GTATCAA26150.039.968711
ATCAACG30250.034.0753483
TCAACGC30650.033.6306464
CAACGCA30750.033.521285
TATCAAC31750.032.6826482
AACGCAG32100.032.1115046
AGAGTAC34350.030.6130611
ACGCAGA33850.030.5532257
CAGAGTA34950.030.18616110
CGCAGAG35600.029.3418248
GCAGAGT37300.028.3742269
AGTACGG34150.027.56167613
GAGTACG34600.027.20321512
TCGTATG809.339467E-525.9645840
GTGGTAT3900.025.649721
ACGGGTA1151.7072907E-623.98423216
TACGGGG17350.023.6472815
GTACGGG39550.023.53699114
GTACCTA2850.022.9962981
Produced by FastQC (version 0.11.5)