FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108551
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26650.040.6849821
GGTATCA10650.037.1679841
ATCAACG31400.034.416133
TCAACGC31600.034.0893944
CAACGCA31900.033.876695
GTGGTAT4900.033.7183231
TATCAAC32900.032.8470042
AACGCAG33200.032.5501946
TGGTATC5000.032.3511622
ACGCAGA34350.031.4675467
AGAGTAC34400.031.43173611
CGCAGAG35300.030.6289778
CAGAGTA35550.030.41495510
GCAGAGT38300.028.1412059
AGTACGG34850.027.07353213
GAGTACG35400.026.9447212
TACGGGG16900.023.43194215
GTACGGG40750.022.81567414
AAGGCGC4150.022.40334323
CCGTTAA1554.182766E-822.20896316
Produced by FastQC (version 0.11.5)