FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578763
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG24910.43040069942273435No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17820.30789805153404765No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTAATATTATCCCACAC16550.28595469993762557No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16470.2845724415693471No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16160.27921619039226764No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTA15670.27074985788656153No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13970.24137686756064225No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12880.222543597292847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11560.19973633421625087No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11470.1981812935519375No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11230.19403451844710184No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11220.19386173615106703No Hit
GATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG10990.18988774334226616No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10550.18228532231673414No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10130.17502846588327173No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC10120.1748556835872369No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9460.16345205204893887No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT9370.16189701138462548No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9330.1612058822004862No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9300.16068753531238175No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9070.1567135425035809No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8940.15446737265512825No Hit
GTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC8880.15343067887891934No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8790.15187563821460598No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8700.15032059755029262No Hit
ATATTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC8640.14928390377408368No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8490.1466921693335614No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG8450.1460010401494221No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7890.13632523157147225No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7590.13114176269042768No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7580.13096898039439286No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTAA7500.12958672202611432No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7430.12837724595387057No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG7420.12820446365783578No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATT7290.12595829380938312No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG7200.12440325314506974No Hit
GGTGATAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT7200.12440325314506974No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7130.12319377707282601No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6940.11991091344816444No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6690.11559135604729397No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6680.11541857375125915No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT6590.11386353308694577No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6420.11092623405435387No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6250.10798893502176193No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC6220.10747058813365747No Hit
CGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC6200.10712502354158783No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACT6100.10539720058123964No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG6060.10470607139710036No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG6040.10436050680503073No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT6010.10384215991692627No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC6000.10366937762089146No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5950.10280546614071737No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGT5940.10263268384468253No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT5930.10245990154864773No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG5900.10194155466054326No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5890.10176877236450843No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC5880.10159599006847363No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT5830.10073207858829954No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCA5830.10073207858829954No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA5810.1003865139962299No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATTTA200.007399686651.6746751
GTGGTAT1600.047.3684541
TGGTATC1700.044.5628172
GTATCAA12400.041.9509471
GGTATCA4700.038.847631
GCCGTTA557.479961E-637.56859215
CCGTTAA557.479961E-637.56859216
TATCAAC14600.035.3783262
TTCGGGG2400.034.571670
TGTCGTA400.002579707234.54233638
GTTAGGG601.3548459E-534.4497831
TAAACAT601.3588615E-534.43490220
CAACGCA15050.034.3205035
TCAACGC15200.033.9818084
ATCAACG15200.033.7552643
AACGCAG15250.033.6445926
AGAGTAC16200.032.09950311
GAAACAA11550.032.05039670
TAGGCAA2008.731149E-1031.9122570
CTCGCTA652.3469096E-531.7915623
Produced by FastQC (version 0.11.5)