FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578763
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG24310.4200337616606452No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17690.30565188168559493No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15290.2641841306372384No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTAATATTATCCCACAC15130.26141961390068125No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14230.24586920725754757No Hit
GACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTA13900.24016739148839855No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12610.21787847529990684No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12250.21165831264265336No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG12170.21027605427437485No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11710.20232806865677316No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11660.20146415717659907No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11360.19628068829555448No Hit
GATTATCACCAGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGG10100.17451011899516727No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9910.17122725537050573No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9700.16759882715377453No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9540.1648343104172174No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTCGGTCCCTC9450.16327926975290405No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9120.15757745398375503No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCT8890.1536034611749542No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8390.1449643463732132No Hit
GTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC8370.14461878178114357No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA8310.14358208800493466No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG8050.13908974830802937No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8030.13874418371595973No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8000.13822583682785528No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7980.13788027223578564No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7760.13407906172301962No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGGCCCTCAGG7710.13321515024284553No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7450.1287228105459402No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7450.1287228105459402No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTAATT7320.12647664069748757No Hit
GGTGATAATCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT7320.12647664069748757No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7270.12561272921731348No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7110.12284821248075638No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA7110.12284821248075638No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7040.12163873640851264No Hit
ATATTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC6870.1187014373759207No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT6800.11749196130367698No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACT6750.11662804982350287No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6630.11455466227108507No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCAC6560.11334518619884133No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6550.1131724039028065No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGGTGATAATCAGGGTGG6520.11265405701470205No Hit
GGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC6460.11161736323849314No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC6420.11092623405435387No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT6400.1105806694622842No Hit
CGATTACCTGTGGGGGAAACAACATTGGAATTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC6380.11023510487021457No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTACTGACTCAGC6330.10937119339004048No Hit
ACCTAGGACGGTCAGCTCGGTCCCTCCGCCGAACATCCAATGATCACTAA6310.10902562879797084No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA6140.10608832976537894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6130.1059155474693441No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6110.10556998287727447No Hit
GCACAGGCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCGGTGTCA6070.1048788536931352No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG6040.10436050680503073No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5990.10349659532485664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1550.048.8224561
GGTATCA6450.045.863521
TGGTATC1700.044.503062
GTATCAA15150.038.143921
CGTATGC400.002633364234.397641
TTTACAC501.89593E-434.3887254
TATCAAC17350.032.9021842
ATCAACG18050.031.6261983
TCAACGC18350.031.4839534
AGGTATC553.3172927E-431.2624762
CAACGCA18550.031.1445065
AACGCAG18550.031.1445066
CGTGCTA450.004682521330.58360910
GTATCCT450.00469441430.5677574
GAAACAA10650.029.58167670
ACGGGAC1401.4551915E-1129.49133716
CAGAGTA19550.029.3907710
CGCAGAG19700.029.3416358
AGAGTAC19500.029.11324311
CCGGACG605.477799E-428.6969429
Produced by FastQC (version 0.11.5)