FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825187
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24550.041.1672131
GGTATCA10200.037.072181
ATATGCG501.7144313E-434.9847354
TATCAAC29300.034.7111632
TCGTTAT400.00257219334.56461737
ATCAACG29350.034.4178923
TATACCG400.002648432734.359365
TCAACGC29650.034.0696534
CAACGCA29750.033.955135
AACGCAG30650.032.958086
ACGCAGA32800.030.6929657
CGCAGAG33150.030.4744038
AGAGTAC33600.030.06626111
CAGAGTA33800.029.88835510
GATCGGA703.9568764E-529.45266311
GCAGAGT36100.028.0792949
AGTACGG26450.027.67102813
GAGTACG26500.027.48915312
TACGGGG13850.027.04094115
TTAACGG2050.025.26807635
Produced by FastQC (version 0.11.5)