FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164992
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA45080.3869554469043564No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44460.38163352194693184No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT30920.26540954787672366No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGAT30810.2644653353842773No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT29970.25725498544196007No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29860.2563107729495138No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28770.24695448552436414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26250.2253234356974125No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25460.21854227325166184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23230.1994005109047959No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23140.1986279734109762No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22620.1941644234466846No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22350.19184681096522552No Hit
CTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT20170.17313423611492612No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTA20020.1718466736252266No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT19280.1654946986760424No Hit
CACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCA18760.1610311487117508No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18750.16094531121243752No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGA18650.1600869362193045No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17770.1525332362797341No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17580.1509023237927814No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGG17210.14772633631818932No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG16900.145065373839477No Hit
GCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16900.145065373839477No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16860.1447220238422238No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG16280.13974344888205242No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15870.1362241114102071No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACACAGTC15850.13605243641158052No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC15170.1302154864582761No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15140.1299579739603362No Hit
GAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCA14790.1269536614843707No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTT14710.12626696148986433No Hit
GTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG14500.124464374004285No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14210.1219750865241993No Hit
TCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACT14210.1219750865241993No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14060.12068752403449981No Hit
CATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA13940.1196574740427402No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13930.11957163654342691No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13850.11888493654892052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13710.1176832115585343No Hit
CATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG13510.11596646157226831No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13510.11596646157226831No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13500.115880624072955No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13350.11459306158325552No Hit
GAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTC13000.11158874910729001No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12870.11047286161621711No Hit
CCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTC12730.10927113662583092No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12390.10635266164917871No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12190.10463591166291271No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11900.10214662418282701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10250.047.4154171
GTATCAA27800.044.0157281
TATCAAC32900.037.151122
TCAACGC32900.037.151124
ATCAACG33000.037.0385363
CAACGCA33400.036.5949635
AACGCAG34500.035.527966
CGCAGAG36550.033.5352868
GTGGTAT3000.033.3197021
ACGCAGA36900.033.1238947
AGAGTAC37050.032.9897911
GCAGAGT39200.031.3560669
CAGAGTA40900.030.05275510
TGGTATC3250.029.6629332
AGTACGG33450.029.54113
GAGTACG33900.029.04729812
GAAATCG500.00719396428.0130660
GTCTAGT500.007769340627.5749241
ACCGATC756.218202E-527.56426427
GTACGGG38050.025.96968314
Produced by FastQC (version 0.11.5)