FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164992
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45510.39064645937482834No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCA39290.33725553480195575No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT29450.2527914354776685No Hit
GTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGAT28450.2442076855463385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27120.2327912981376696No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27070.23236211064110313No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25500.21888562324891503No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25470.2186281107509751No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23930.20540913585672693No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23870.2048941108608471No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCGCCGCCGAAAGTG23790.20420741086634073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21680.18609569851123442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21350.18326306103389553No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTA19010.16317708619458332No Hit
CTCCTGTGGGGCCAGTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT18400.15794099873647202No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT18130.15562338625501293No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17700.15193237378454102No Hit
GCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGA17270.14824136131406912No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAATTTATTACTGTCAGCAGCGTAGCAACTGG17110.1468679613250563No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16730.1436061363511509No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16720.14352029885183762No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16590.1424044113607647No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16000.13733999890128No Hit
GCGTAGCAACTGGCCTCTCACTTTCGGCGGCGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15770.1353657364170741No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15640.13424984892600123No Hit
GAGTGAAGTCTGTCCCAGACCCAGTGCCACTGAACCTGGCTGGGATGCCA15150.13004381145964952No Hit
CTGCTACTCTGGCTCCCAGATAGCACCGGAGAAATTGTGTTGACACAGTC15130.1298721364610229No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14850.1274686864802505No Hit
CACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCA14800.127039498983684No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14690.1260952864912377No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14690.1260952864912377No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14510.1245502115035983No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14300.12274762401801902No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14250.12231843652145251No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14040.12051584903587322No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAG13620.11691067406471461No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13420.11519392407844861No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13050.11201793660385652No Hit
GTCAGAGCGTTAGCAGTCACTTAGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG12700.109013624127891No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12640.10849859913201121No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12590.1080694116354447No Hit
CATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG12430.10669601164643192No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTT12330.1058376366532989No Hit
GTTGCTACGCTGCTGACAGTAATAAATTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT12260.10523677415810581No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11980.1028333241773334No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11950.1025758116793935No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11880.10197494918420041No Hit
TCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCACT11740.10077322419381421No Hit
CATCTATGATGCATCCATCAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCACTGGGTCTGGGACAGACTTCA11710.10051571169587431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)