FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403425
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGGAGAAGTCCTTACTACTGGGGCTGGGTCCG40710.2900760639150649No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35580.25352263213210535No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31850.22694479576749735No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC31300.223025811853145No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG30210.21525909827742845No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29970.21354899620571102No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29720.21176763988100542No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACAAAACCACCCAGG26310.1874699396120206No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26180.18654363432317367No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25460.18141332810802147No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25100.17884817500044534No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGGAGAAGTCCTTACTACTGGG24540.17485793683310472No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23770.1693713593530114No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGTC23260.1657373924506119No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22280.15875447565776582No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC22070.1572581363450131No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21220.15120152484101396No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21170.15084525357607284No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20880.1487788802394143No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20550.14642748989080284No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20050.1428647772413916No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACAAAACCACCCAGGG19680.14022836988082726No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19590.13958708160393324No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19140.1363806402194631No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18760.13367297860591054No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17810.12690382457202914No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17670.125906265030194No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACAAAACCACCCAGGGAATTATCCCATGTT17140.12212978962181804No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG16820.11984965352619485No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT16730.11920836524930083No Hit
GTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTC16690.1189233482373479No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTTATGACCGACCCTCAGGGATTCCTGACCGATTCTCTGG16670.11878083973137148No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA16650.11863833122539502No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC16590.11821080570746567No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCCGGGAAGTATGTA16490.11749826317758341No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16080.11457683880506618No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA15450.11008782086680798No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGCGATGACCAGGGTGG15130.10780768477118478No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC14870.10595507419349091No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG14850.10581256568751449No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14810.10552754867556156No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14800.10545629442257334No Hit
CTACTGGGGCTGGGTCCGCCAGCCCCCAGGGAAGGGACTGGAGTGGATTGGGAGTTATTGGTACAGTGGGAACAC14720.10488626039866754No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14510.10338992108591481No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14450.10296239556798546No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA14410.10267737855603257No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14280.10175107326718562No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT14270.10167981901419741No Hit
GTGATATAGTGACTCGACTCTTGAGGGACGGGTTGTAGTAGGTGTTCCCACTGTACCAATAACTCCCAATCCACT14220.10132354774925628No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14160.10089602223132693No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14150.1008247679783387No Hit
GAAATAAACGGCACTGTCCGCGGCGGTCACAGACTTCACCTTCAGGGAGA14130.10068225947236227No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14090.10039724246040935No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC14050.10011222544845647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG851.8189894E-1244.7554740
GTGGTAT3250.043.4949871
GTCGTAT404.9387894E-543.218939
GTATCAA28100.038.4040261
TATCAAC30200.035.823442
ATCAACG32100.033.9177253
TCAACGC32350.033.6556054
TATACAC1550.033.3429643
ATACACA1550.033.3429644
ACGAGAC1350.033.1806722
CGTATGC1056.2391337E-1032.95578441
CAACGCA33600.032.5060845
AACGCAG34550.031.6122866
ACGGGTA1450.030.8912516
CTTATAC1600.030.1680181
CGCAGAG36600.029.9368528
AGAGTAC36750.029.81572511
CTCGTAT703.794869E-529.63581539
CAGAGTA37300.029.37608110
GCAGAGT38750.028.2758439
Produced by FastQC (version 0.11.5)