FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998401
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19570.19601342546732223No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAA18670.1869990114192594No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15350.15374583959751642No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA13030.1305086833847322No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11960.11979154668314636No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA11930.1194910662148776No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10880.10897424982547092No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10860.10877392951329176No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATACCAGCGGCCCTGGT10760.10777232795239587No Hit
GTGTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCA10670.10687088654758961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA24000.036.4859475
ATCAACG23750.036.4345363
TCAACGC24000.036.1986544
AACGCAG24650.035.523846
AGAGTAC26600.032.406111
CGCAGAG27500.031.8454698
ACGCAGA28750.030.4578327
CAGAGTA28750.030.2225510
GCAGAGT29250.030.0595669
GTATCAA29400.029.6879981
AGTACGG22750.028.19031513
TATCAAC31700.027.7321822
GTATATA756.163808E-527.5992771
AATACCG500.00776194727.5799275
GAGTACG23550.027.23268312
GAGTACT9000.024.51917612
AGTACTT9050.024.38370913
GTACGGG26700.024.01983814
GTATTAT2900.023.7924821
ATTGCGT2200.023.505626
Produced by FastQC (version 0.11.5)