FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998401
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18770.18800061298015527No Hit
GTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCAAGGGACCGAGGTGGAAATGAAACGAA18240.18269212470740714No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14510.1453323864859911No Hit
CAGTAATACACTGCAAAATCTTCAGACTCCAGGCCGCTGATGGTGAGACTGAACTCTGTCCCAGACCCACTGCCA11670.11688690215654832No Hit
GTCTGGGACAGAGTTCAGTCTCACCATCAGCGGCCTGGAGTCTGAAGATTTTGCAGTGTATTACTGTCAGGAGA11570.11588530059565244No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11340.11358161700559194No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11110.11127793341553142No Hit
GTGTATTACTGTCAGGAGACTACTAACTGGCCTCCGAAGACGTTCGGCCA10380.10396624202099157No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10130.10146223811875188No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10020.10036047640176642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5100.034.4363561
TCAACGC25200.034.2893944
ATCAACG25350.034.08653
CAACGCA25650.033.6878245
AACGCAG26450.032.6738246
AGAGTAC28250.031.21698611
CGCAGAG28200.030.9058658
GTATCAA29350.030.0364781
GCAGAGT29550.029.7270779
CAGAGTA29900.029.49431210
AATCGTC605.226907E-428.93029247
TAAGTTA500.006483447328.6156167
ACGCAGA30650.028.4339877
TATCAAC31850.027.8866882
CGTAACG500.00770384227.62242133
ACGTAAC500.007707619627.61964632
AGTACGG26850.025.65991413
GAGTACG27250.025.4096712
GACGTAA851.4390987E-424.36293431
TTAACGG1001.5481583E-524.1805535
Produced by FastQC (version 0.11.5)