FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709090
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22370.3154747634291839No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21740.30659013665402135No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17060.24059005203852823No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT15550.2192951529425038No Hit
GTATGATATTCTCCCTCCGGGATTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15510.21873104965519186No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15350.21647463650594423No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15240.2149233524658365No Hit
ATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA15050.21224386185110491No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAATAGCTAT15000.21153873274196508No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13600.19179511768604832No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG13400.18897460124948878No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13260.1870002397438971No Hit
GTGTATTATTGTGCGAGAGATCTGGGATACACCTTTGGTTCAGGTCCCTT13160.18558998152561734No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG13150.18544895570378936No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12570.17726945803776673No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAATAGCTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12340.17402586413572324No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12180.1717694509864756No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11100.15653866222905413No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10940.1542822490798065No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10890.15357711997066664No Hit
CAATAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA10550.14878224202851542No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAG10520.1483591645630315No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT10520.1483591645630315No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAACAGCCTGA10390.1465258288792678No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10330.14567967394829995No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10310.14539762230464398No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10240.14441044155184815No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC10190.14370531244270826No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10090.14229505422442848No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT9960.14046171854066478No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9820.13848735703507312No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9800.13820530539141715No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9770.13778222792593323No Hit
GGGCTGGAGTGGGTGGCCACCATAAAGGAAGATGGAAGTGAGAAATACTA9760.13764120210410527No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9730.13721812463862135No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9670.13637196970765347No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9610.1355258147766856No Hit
ATATCATACTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC9600.13538478895485764No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA9590.13524376313302966No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA9570.13496171148937372No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAATCCC9530.13439760820206179No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9520.13425658238023383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9390.13242324669647013No Hit
CCATAAAGGAAGATGGAAGTGAGAAATACTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACG9370.13214119505281416No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9340.13171811758733024No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGGGACCTGAACCAAAGGTGTATCCCAGATCTCTCGCACAATAATACACAG9270.1307309368345344No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAGTTGGTATCAGCAGAAA9260.13058991101270642No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9180.1294617044380826No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA9170.12932067861625463No Hit
GCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTC9030.12734631711066297No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT8920.12579503307055523No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8910.12565400724872725No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC8840.1246668264959314No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG8570.12085912930657604No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8390.11832066451367244No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA8390.11832066451367244No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCA8330.1174745095827046No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA8220.11592322554259685No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT8120.11451296732431708No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA8070.1138078382151772No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC7970.11239757999689742No Hit
GAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGGTTTTCCTTGT7880.11112834760044564No Hit
GTATTATTGTGCGAGAGATCTGGGATACACCTTTGGTTCAGGTCCCTTTGACTACTGGGGCCAGGGAACCCTGGT7870.11098732177861766No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7870.11098732177861766No Hit
AGTCAGGACATTAGCAACTATTTAAGTTGGTATCAGCAGAAACCAGGGAA7740.10915398609485398No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC7740.10915398609485398No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA7670.10816680534205814No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7540.10633346965829443No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7530.10619244383646645No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACC7470.10534628890549859No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG7470.10534628890549859No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7460.10520526308367062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7450.10506423726184264No Hit
CTATGTGGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAA7380.1040770565090468No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC7350.10365397904356288No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7200.10153859171614323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCGA200.007147434652.13115354
ATCGGTT200.00723822651.9650144
GATCGGT200.007248366751.9466243
ACCGCTT200.00729718951.85850537
ACTGCGC306.2886364E-446.0313848
GTGGTAT1100.043.9142531
GTATCAA8850.040.157261
TATCAAC10450.034.3292772
ATCAACG11050.032.4652443
ATACTGG652.3206487E-531.8454066
TTCGGGG9200.031.4657770
TCAACGC11300.031.4417294
CAACGCA11450.031.0298295
AACGCAG11550.030.7655096
CACTGCG450.00460929630.6832587
AAAAGTT550.00493751330.25390269
CGCAGAG12250.029.3097788
CAGAGTA12450.029.12034610
AGTACTT1900.029.07655513
AGAGTAC12850.028.21387511
Produced by FastQC (version 0.11.5)