FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427868
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGAAGCTGTATAAAACATT43470.3044399062098177No Hit
ATATTATACTCCTCCCCTGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT37620.26346973249628114No Hit
CTCCTAAGCTGCTCATTTCCTGGGCATCTAACCGGGAATCCGGGGTCCCT35310.24729176646580775No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34020.2382573179033356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33670.23580611092902143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25870.18117921264430606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25740.1802687643395608No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC25000.1750862124510109No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23810.16675210873834276No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23690.1659116949185779No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23490.16451100521896983No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23320.16332041897430294No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22200.15547655665649765No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21730.15218493586241866No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21560.1509943496177518No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTCACAGACCTCACCTTCAGGGATA20350.14252017693512287No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGTT19890.13929859062602426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19310.1352365904971608No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGCC17880.12522165914496297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17670.1237509349603745No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA17630.12347079702045287No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC17530.12277045217064883No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17090.11968893483151102No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17090.11968893483151102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16920.11849834858684416No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC16380.11471648639790233No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16380.11471648639790233No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16300.11415621051805909No Hit
GTCAGGGACCCCGGATTCCCGGTTAGATGCCCAGGAAATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTA16280.11401614154809828No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15980.11191510699868615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15740.11023427935915645No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTATTACTGGGCCTGGATCCG15600.1092537965694308No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15530.10876355517456796No Hit
ATTATACTCCTCCCCTGGCGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15490.10848341723464636No Hit
GTATAAAACATTCTGGCTGGACTTGCAGTTGATGGTGACCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA15220.10659248614017541No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGAAGCTG14530.10176010667652753No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14350.10049948594688025No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14300.10014931352197821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4500.039.0234871
GGTATCA10050.038.029911
GTATCAA21800.037.2755361
TGGTATC5300.033.1250272
TATCAAC25050.031.881752
CAACGCA25500.031.320235
ATCAACG25500.031.3191343
TCAACGC25850.031.028254
CGGTTAA450.00452614430.79975945
TACGGAT8400.030.33261770
AACGCAG26800.029.9315156
AGAGTAC28700.028.08863311
CAGAGTA29200.027.6076610
CGCAGAG29650.027.1867548
GCAGAGT31150.025.8785089
AGTACGG29350.025.23637613
ACGCAGA32350.025.0215457
GAGTACG30000.024.57475312
CGAACTA1202.4704495E-622.98719829
CAGTTCG14250.022.46496870
Produced by FastQC (version 0.11.5)