FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC86900.5140068341023719No Hit
GTATAATAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT78220.4626652999250579No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53320.31538370994635756No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATTCACAAGGTGT47180.27906608093152946No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38900.2300905160711423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36890.2182015202535846No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC36170.21394277548311613No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34020.201225690404634No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33100.19578396097570208No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31350.18543284521414685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30950.18306687589721993No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGCCTCAGGGAGA29790.17620556487813188No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG29620.17520002791843794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29490.17443108789043668No Hit
GTCTCTGGTGGCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG29200.17271576013566467No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28150.16650509067873154No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26380.15603567645132993No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT25810.15266417017470907No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25580.1513037378174761No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA24570.14532966529223565No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24060.14231305441315387No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACT23000.13604323572329752No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA22960.13580663879160482No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22680.134150460269756No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22610.13373641563929378No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22490.1330266248442157No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22380.13237598328206082No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22220.13142959555529002No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22190.1312521478565205No Hit
CAGTATAATAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21970.1299508647322107No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21800.12894532777251677No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21660.12811723851159237No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC20970.12403594143989344No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20870.12344444911066171No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20780.12291210601435315No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20780.12291210601435315No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20620.12196571828758239No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19630.11610994422818828No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19520.11545930266603338No Hit
GTCATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACTCCAGTAGTAATCATCA19500.11534100420018702No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19390.11469036263803212No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19330.11433546724049308No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC19320.11427631800756992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19040.11262013948572108No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18830.11137800559433446No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18790.11114140866264177No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGATGATTACTACTGGA18740.1108456624980259No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGCTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC18630.110195020935871No Hit
GTCTCCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTT18280.10812479778355993No Hit
GTGTATTACTGTGCCAGAGGTCGTCGGGAAGCTTATCACCATGATGGTTA17760.10504903767155496No Hit
GTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT17620.10422094841063052No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATTCACAAGGTGTCTTATTTACAAAGTGGGGTCCCAT17480.10339285914970611No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17180.10161838216201094No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17120.1012634867644719No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17010.100612845202317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATCG400.00983023848.0933670
GTGGTAT4200.047.4562571
GTATCAA27750.037.5227281
TATCAAC31850.032.7693442
ATCAACG33050.031.475653
TCAACGC33050.031.371774
CAACGCA33250.031.0798135
AACGCAG33900.030.382616
TTCGGGG13550.030.2875870
TCGTATG953.085006E-729.17984840
CGTATGC854.270716E-628.5455541
CCGTTAG500.0078854927.4919011
CGCAGAG37750.027.3765548
AGAGTAC37900.027.2690111
TGTTAGA10750.026.84280670
CGGCCAA24250.026.77225122
TGTGTAT2500.026.09490412
GTTCGGC24850.025.9791519
GCAGAGT39800.025.9664579
TTCGGCC24850.025.84172820
Produced by FastQC (version 0.11.5)