FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690639
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC82320.4869164854235588No Hit
GTATAATAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT72050.42617022321146025No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51280.3033172664300303No Hit
GAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATTCACAAGGTGT41870.2476578382493247No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35260.2085601952871074No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34760.20560273364094878No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31370.1855511436799932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30770.18200218970460283No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30650.18129239890952475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29870.17667875874151726No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28320.16751062763842547No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGCCTCAGGGAGA27540.16289698747041798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26760.1582833473024105No Hit
GTCTCTGGTGGCTCCATCAGCAGTGATGATTACTACTGGAGTTGGATCCG26390.15609482568425312No Hit
CCTTCCACCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG24710.14615775455316007No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24500.14491562066177344No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23830.14095262205592085No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG23480.13888239890360982No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23360.13817260810853174No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT23210.13728536961468416No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23000.13604323572329752No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTA22700.13426875873560234No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22460.1328491771454462No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22400.13249428174790717No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22270.1317253417199059No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGGTGACT21570.1275848954152838No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20540.12149252442419702No Hit
CAGTATAATAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20470.1210784797937348No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20330.12025039053281036No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA20330.12025039053281036No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGGTGGTTGGCCTGGTATCAGCAGAAA19980.11818016738049933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19440.11498610880264798No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19370.11457206417218578No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGC19180.1134482287466455No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19070.1127975871844906No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18780.1110822594297186No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18720.11072736403217956No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18670.1104316178675637No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18470.10924863320910022No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18350.10853884241402215No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18190.10759245468725138No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGCTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17960.10623202233001841No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17940.10611372386417207No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17780.10516733613740131No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17110.10120433753154873No Hit
GTCATAGATGTACCCAATCCACTCCAGGCCCTTCCCTGGGGGCTGGCGGATCCAACTCCAGTAGTAATCATCA17070.10096774059985604No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC17030.10073114366816334No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17020.10067199443524018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4300.047.0791931
GTATCAA29400.040.1473161
TATCAAC34150.034.4504362
ATCAACG34100.034.400363
TCAACGC35050.033.467974
CAACGCA35300.033.328115
AACGCAG35950.032.918286
CGCAGAG39400.030.1335988
AGAGTAC39100.029.83912811
ACGCAGA41250.028.6140887
GCAGAGT42250.028.0197019
AGTACGG38100.025.12830513
ACGGGTA1101.2092587E-624.95488516
TGTTAGA10900.024.93050670
GAGTACG38750.024.706812
CGGCCAA25800.023.82531522
TCCTATA2500.023.3238472
GTTCGGC26400.023.01209419
TCAAACG27050.022.99188445
AGCGCTG4650.022.8665165
Produced by FastQC (version 0.11.5)