FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1926884
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42990.22310632087868287No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC38850.2016208552253275No Hit
CTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA32620.16928886222522996No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31240.16212704034077818No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30010.15574367735681027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28850.1497235951930682No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGGTCAGGTTCATGGAGA28600.1484261636922617No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC28180.1462464787709068No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27830.14443007466977772No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27090.14058967742739054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26200.13597082128451946No Hit
TTATTACTGCATGCAAGCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA25700.1333759582829065No Hit
GGCCAGGCCAGTCTCCAAGGCGCCTCATTTATAAGGTTTCTAACCGGGAC24890.12917228022029348No Hit
GCTACACACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT24660.12797864323955152No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG24630.12782295145945474No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATGAGGCGCCTTGGAGACTGGCCTGGCCTCTGCTGAAA24310.12616223913842245No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCTGGCACTGATTTCACATTGAA24000.12455342407742241No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22940.11905231451400292No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA22730.11796247205332548No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21890.1136031022106157No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21770.11298033509022858No Hit
GTGCCAGACCCACTGCCGCTGAATCTGTCTGGGACCCCAGAGTCCCGGTT21460.11137152002922852No Hit
GTCTGGCACTGATTTCACATTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAG20960.10877665702761558No Hit
GGCCTGGCCTCTGCTGAAACCAATTCAAGTAGGTGTTTCCATCATTGTGT20930.1086209652475188No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAATGTGAAATCAGTGCCAGACCCACTGCCG20340.10555902690561549No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20300.10535143786548645No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20160.10462487622503482No Hit
GTCTCCACTCTCCCTGCCCGTCACTCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTACA20160.10462487622503482No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19540.10140724610303474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19530.10135534884300249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)