FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285923
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATCGTTATCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT64070.498241341044526No Hit
CGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC51320.39909076982058805No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37280.28990849374340455No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33850.2632350459553177No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27380.212920991381288No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT27350.2126876959195846No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27210.21159898376496883No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25930.20164504406562445No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24160.18788061182512483No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC24130.18764731636342144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23350.18158163435913346No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22900.17808220243358272No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22070.1716276946597891No Hit
AGTCAGGACATTAGCAATTATGTAGCCTGGTTTCAGCAGAAACCTGGGAA21490.1671173157335237No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21360.16610636873280904No Hit
GATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA20310.15794102757319062No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG19550.1520308758767049No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAACAGTATTATCGTTATCCGGTCACCTT19550.1520308758767049No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19230.1495423909518688No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19120.1486869742589564No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT18230.14176587556175602No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18200.14153258010005262No Hit
GTCTGCATCTGTAGGAGACAGAATCACCATCACTTGTCGGGCGAGTCAGG18160.14122151948444814No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGACAGAATCACCATCACTT18130.14098822402274475No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17980.13982174671422784No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCAGGTTTCTGCTGAAACCAGGC17820.13857750425180979No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17780.13826644363620527No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17580.13671114055818273No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT17460.1357779587113692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17440.13562242840356695No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT17300.13453371624895114No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17230.13398936017164326No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16370.12730155693614625No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16360.12722379178224513No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15830.12310223862548535No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA15800.12286894316378197No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC15640.12162470070136391No Hit
GTAATAAACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCCTCAGGGAGA15540.12084704916235266No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG15470.12030269308504475No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15420.1199138673155391No Hit
GTTTATTACTGTGCGAGGAAGTCGGCCTATTTCTATGACAGTAGTGGTTA14980.1164922005438895No Hit
GTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGA14970.11641443538998836No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG14450.11237064738712972No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA14430.11221511707932745No Hit
GGCTGGTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAA14260.11089310946300829No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14160.110115457923997No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14050.1092600412310846No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13630.10599390476723723No Hit
GTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCACTTTGCAAACT13610.10583837445943498No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13530.10521625322822595No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13440.10451636684311581No Hit
GTCCCTGATCTATGGTTCATCCAGTTTGCAAAGTGGGGTCCCATCAAAGT13410.10428307138141242No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13370.1039720107658079No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC13290.10334988953459888No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13040.10140576068707069No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12910.10039481368635604No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12880.10016151822465265No Hit
GATTTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGCGAACAGTATTATCGTTAT12870.10008375307075151No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCGGATAACGATAATACTGTTCGCAGTAATAAGTCGCAAAATCTTCAGG12860.10000598791685038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGAG350.005183384856.54163470
GTGGTAT3250.045.5057561
TGGTATC3500.041.2566152
GGTATCA7900.038.3121761
GTATCAA17450.037.448931
TGTTAGA9300.035.4651870
GTATACT450.004693079330.5723441
ATCAACG21550.030.4718233
TATCAAC21500.030.3827762
TCAACGC21750.030.191624
CAACGCA22200.029.5796285
AACGCAG22650.029.1505456
TTCGGGG9050.026.96921270
TATTGAT8100.026.87472770
AGAGTAC24500.026.68838111
CGCAGAG24650.026.664558
ATACCCG658.834829E-426.4465475
CAGAGTA25400.025.87821810
ATTAGAG1208.810821E-825.7853853
GCAGAGT25600.025.6760449
Produced by FastQC (version 0.11.5)