FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120303
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTG29940.2672491281376556No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG29450.26287531141128784No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26190.2337760409460655No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC24530.2189586210159216No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACT23660.21119286478747268No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21940.1958398754622633No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCT21800.19459021354044395No Hit
CCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCA21060.18798485766797018No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC19720.17602380784484198No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19550.1745063612254899No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19370.17289965304029356No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18300.16334866549495985No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17370.1550473398714455No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16800.14995943061832379No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG16530.1475493683405293No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16460.1469245373796196No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG15980.14263998221909607No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15660.1397836121120804No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15320.13674871887337622No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15120.1349634875564914No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14790.1320178558836315No Hit
GGATAAGAATCGGCCCTCTGGGATCCCAGAGCGATTCTCTGGCTCCAATCTGGGGAATACGGCCACCCTGACCAT14100.1258588078403789No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGG14090.12576954627453465No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG14080.12568028470869042No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13660.12193129894323232No Hit
GGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGG13600.12139572954816688No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13560.12103868328478991No Hit
CCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGT13500.12050311388972447No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13400.11961049823128206No Hit
GTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCT13300.11871788257283968No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13030.11630782029504518No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13000.11604003559751246No Hit
CTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT12840.11461185054400462No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGC12730.11362997331971797No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12640.1128266192271198No Hit
GTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCAGAGCG12550.11202326513452164No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGC12480.11139843417361196No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12460.11121991104192348No Hit
GGGTGGCCGTATTCCCCAGATTGGAGCCAGAGAATCGCTCTGGGATCCCA12350.11023803381763685No Hit
CTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGC12290.10970246442257138No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGAC12180.10872058719828474No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT12040.10747092527646539No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11980.10693535588139995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)