FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120303
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTG29540.26367866550388597No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC26590.23734650357983508No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG26040.23243711745840187No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25150.22449283809826448No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCT22690.20253449290058137No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACT22630.2019989235055159No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20660.18441439503420057No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19860.17727346976666136No Hit
CCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCA18850.16825805161639307No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC18810.1679010053530161No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17860.15942115659781328No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17660.15763592528092846No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17230.15379767794962612No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16250.14505004449689057No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16210.14469299823351361No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC15630.13951582741454768No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG15430.13773059609766286No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15310.13665945730753198No Hit
GGGTGGCCGTATTCCCCAGATTGGAGCCAGAGAATCGCTCTGGGATCCCA15060.13442791816142596No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14600.1303218861325909No Hit
GTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCT14000.1249661921819365No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14000.1249661921819365No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13970.12469840748440376No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGG13730.12255612990414201No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGC13610.1214849911140111No Hit
GGATAAGAATCGGCCCTCTGGGATCCCAGAGCGATTCTCTGGCTCCAATCTGGGGAATACGGCCACCCTGACCAT13580.1212172064164784No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13550.12094942171894567No Hit
GTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCAGAGCG13270.11845009787530694No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12870.11487963524153733No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12870.11487963524153733No Hit
CTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGC12770.11398701958309494No Hit
GGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGG12550.11202326513452164No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG12460.11121991104192348No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11990.10702461744724419No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGC11980.10693535588139995No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT11930.10648904805217875No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11850.10577495552542482No Hit
CTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT11780.10515012456451514No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGAC11720.1046145551694497No Hit
CCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGT11610.10363267794516307No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT11580.10336489324763033No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACATTTTTACTT11570.10327563168178609No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11430.10202596975996672No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11410.10184744662827824No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11310.10095483096983585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.043.202791
GTATCAA27550.040.9579961
GTGGTAT3650.039.5861171
TATCAAC30550.036.816752
TGGTATC4100.035.235012
ATCAACG32250.034.6627123
TCAACGC32500.034.396084
CAACGCA32850.034.0296065
AACGCAG33850.033.0257726
AGAGTAC35000.032.06324811
CAGAGTA35850.031.49507110
CGCAGAG35700.031.4331518
ACGCAGA35950.031.2089867
CGACCGG850.00436979931.01883770
GCGTTAA450.004434891530.92765850
GCAGAGT37150.030.1149739
AGTACGG30850.028.11922313
GAGTACG31050.027.938112
TACGGGG12350.026.19637915
ACGGGTA809.736056E-525.80627316
Produced by FastQC (version 0.11.5)