FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357451
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAAT15600.4364234538440234No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT13890.3885847290957362No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA12360.34578165958411083No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11470.3208831420250608No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTC10530.2945858313447158No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC10310.288431141611018No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10060.28143717600454327No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG9510.2660504516702989No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAG9470.2649314171732629No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGGAGG9320.26073503780937807No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT9160.25625889982123423No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC8980.2512232445845724No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG8960.25066372733605446No Hit
GATTATTACTGCTGCTCAATTGCAGGAATTACCACTTGGGTCTTCGGAACTGGGACCCAGGTCACCGTCCTAGGT8600.24059241686273086No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGT8580.24003289961421287No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG8240.2305211063894072No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG7720.21597365792793977No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC7640.21373558893386788No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGTATTATAATCTTGTCTCCTGGTACCAACAACA7620.21317607168534988No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC7280.20366427846054422No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7260.20310476121202628No Hit
GTCCCCAAACTCATCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGT7090.19834886459962348No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC6770.18939658862333578No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG6620.18520020925945094No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT6120.1712122780465015No Hit
GTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGACAAGATTATAA5810.16253976069447282No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC5730.1603016917004009No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT5630.157504105457811No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG5570.15582555371225706No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC5220.14603400186319243No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT5170.1446352087418975No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT5020.14043882937801264No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGA4900.1370817258869048No Hit
CCCTGAGAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGC4780.1337246223957969No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC4760.13316510514727892No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA4750.13288534652301995No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC4720.13204607065024299No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG4650.13008776028043004No Hit
GCCTGGAGCCCAGAGATTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC4490.1256116222922862No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA4450.12449258779525026No Hit
CTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGC4360.12197476017691934No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC4300.12029620843136543No Hit
CCCCTGAGGGCCGCTTACTGCCCTCATAAATGATGAGTTTGGGGACTTTGCCTGGGTGTTGTTGGTACCAGGAGA4260.11917717393432946No Hit
TTCTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAACCCCTGAGGGCCGCTTACTGCCCT4150.11609982906748058No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG4140.1158200704432216No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC4090.11442127732192663No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC4070.11386176007340866No Hit
CAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTGCTGC4050.11330224282489068No Hit
TCATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCT4030.11274272557637272No Hit
CCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGAATCTCTGGGCTCCAGG4030.11274272557637272No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA3960.11078441520655977No Hit
GTATCAACGCAGAGTACGGGGGGGTCACAAGAGGCAGCGCTCTCGGGACGTCGCCACCATGGCCTGGGCTCTG3900.10910586346100586No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT3840.1074273117154519No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG3820.10686779446693394No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG3800.10630827721841594No Hit
GCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTCTCAGGG3780.10574875996989797No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT3750.104909484097121No Hit
GTTCCGAAGACCCAAGTGGTAATTCCTGCAATTGAGCAGCAGTAATAATC3730.10434996684860302No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA3680.10295117372730808No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT3660.1023916564787901No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT3590.10043334610897717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)