FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48344
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT5251.0859672348171439No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT890.1840973026642396RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT750.15513817640244912RNA PCR Primer, Index 29 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT610.1261790501406586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11790501406586133No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.11169948700976336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.11169948700976336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.10963097799106404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.10756246897236472No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.10342545093496608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG100.008920789134.0042470
CGTGGAA205.22577E-4100.5031870
ATCCGCC150.001443949877.9209967
TACCGAG150.001832182673.390755
GTACCGA150.001866285273.05093454
CGTACCG150.00188351372.8822253
ACCGTAC150.001892171272.7981651
TTCCAAC150.001927101372.4638446
CTGTTGT150.002127888470.6786140
AGCGATA300.002614447167.0021270
CAGTTGA150.002812753465.88309525
GAACAGT150.002883339565.4730322
GAGTAGC150.002895231165.405181
AGATATC150.00290715965.337485
ACCATGG150.00290715965.337488
TAGCACC150.00290715965.337484
GTAGCAC150.00290715965.337483
GTGAGAC150.00290715965.3374811
AGTAGCA150.00290715965.337482
TGAGACC150.00290715965.3374812
Produced by FastQC (version 0.11.5)