FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48344
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13432.7780076121131887No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.12824755915935793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.11790501406586133No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.10756246897236472No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.10342545093496608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGAT100.009572461130.8891170
CCACATG100.009572461130.8891170
AGTCCAA150.002783985366.0549248
AAGTCCA150.002783985366.0549247
TGCAGCC200.002969863664.95550569
TCAAGCG200.00550848355.58982561
AGAAGTG200.00698142852.36281239
TGCAGCG200.00722687351.90797837
GGCGATT200.008516544549.79921725
GGTGTGA200.008729177549.4899061
TGTGAAG200.008729177549.4899063
TTCATTC200.008729177549.4899062
CGGGTGG400.003185014533.02746217
ATGCTGG400.003185014533.02746217
GGTATCA3250.030.4553281
GTATCAA6150.028.9697021
AGAGTAC6600.028.023311
CAGAGTA6650.027.81259710
GCAGAGT6700.027.6050439
TACGGGC856.0564307E-627.19908515
Produced by FastQC (version 0.11.5)