FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1826679
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69750.3818404875733503No Hit
GTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC57330.31384824591512794No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52200.2857644939258622No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCA49750.272352175724361No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47200.25839241596361484No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44800.24525381854173614No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40580.22215178474159938No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC40420.22127587824680747No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39910.2184839262946582No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG39550.2165131366813764No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38920.21306425485813327No Hit
GTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGTCA38320.20977960550266359No Hit
GTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGTCTCGCATA37500.205290584716855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37480.205181096405006No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35940.19675049639263387No Hit
GTCTGGGACCCCAGAGTCCCGGTGAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGATGAAA35790.19592933405376645No Hit
GTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCACTGATTTCACACTGAA35470.1941775210641826No Hit
GTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT34680.1898527327461475No Hit
GGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGA34260.18755347819731874No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGT33740.18470678208924501No Hit
GTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTG33690.18443306130962253No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33070.1810389236423039No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32800.1795608314323425No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG32400.17737106519536272No Hit
GTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT31910.1746886015550625No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACTCTCC31340.17156818466736629No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31220.17091125479627237No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTCCACT30340.16609376907491682No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30310.16592953660714335No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29310.16045512101469386No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC29220.15996242361137342No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG28930.1583748430895631No Hit
ATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGAGAGTTCCTTCTTGGACGTGTCCACGGC28910.15826535477771408No Hit
GCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGGCCAGTATGAACCTTGCATG28010.15333838074450956No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGA28010.15333838074450956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG27890.15268145087341564No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT27820.15229824178194418No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTGTGATCTCCAACTTGGTCCCTCCGCCGAAAGTGAGCCCCGGAGG27400.1499989872331154No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27250.14917782489424797No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG27240.14912308073832348No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26920.14737126774873965No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26420.1446340599525149No Hit
ATGTATTATAGTGGGCTCACCCACTACAAGCCGTCCCTCCAGAGTCGAGT26260.143758153457723No Hit
CTACAAGCCGTCCCTCCAGAGTCGAGTCACCATGGCCGTGGACACGTCCAAGAAGGAACTCTCCCTGAAGTTGAA26150.14315596774255357No Hit
CTATAATACATAGTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGAATCCAGACCCAGTAATAACTGTTC25880.1416778755325922No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25490.1395428534515369No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25270.13833848202119803No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24960.1366414131875387No Hit
CACTTGTACTGTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGG24830.13592973916052026No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG24670.13505383266572835No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24400.133575740455767No Hit
GTGTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGC24110.13198815993395666No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23300.12755388330407258No Hit
CTAGTACACTGAACGCCGGAAAAACTCGCACAATAATACACAGCCGTGTC22890.1253093729111683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22830.12498090797562134No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA22810.12487141966377234No Hit
GTCTCTGGTGACTCCATCGACTGGAACAGTTATTACTGGGTCTGGATTCG22680.12415974563675392No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22520.123283839141962No Hit
CTCCCTGCCCGTAACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA22130.12114881706090669No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22110.1210393287490577No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22070.12082035212535973No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21940.1201086780983413No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21590.118192632640984No Hit
ATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACACGAACTGTGGCTGCACCAT21560.11802840017321051No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT21370.11698826121064512No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCCG21250.11633133133955117No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21250.11633133133955117No Hit
GTATTGACTCAGTCTCCACTCTCCCTGCCCGTAACCCTTGGACAGCCGGC21240.11627658718362668No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20920.11452477419404285No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20450.1119517988655916No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20260.1109116599030262No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20190.11052845081155474No Hit
CAATAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAACTTCAGGGA20150.11030947418785676No Hit
GTGTACTAGAACCACCTGCTTTGGGGGGAACGACCCCTGGGGCCAGGGAATCCTGGTCATCGTCTCCTCAGCCTC20120.11014524172008329No Hit
GCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGAGCCACTGCC19980.10937882353714035No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19480.10664161574091562No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC19310.10571096509019921No Hit
GGCCAGTATGAACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTC19170.10494454690725628No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19170.10494454690725628No Hit
GTATTATTGTGCGAGTTTTTCCGGCGTTCAGTGTACTAGAACCACCTGCT19100.10456133781578483No Hit
CGCATACAGTGATGGAAACACCTTCTTGAATTGGTTTCATCAGAGGCCAG19000.10401389625653988No Hit
CACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGCTCAGGCAC18830.10308324560582345No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18750.1026452923584275No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGAAATTGTATTGACTCAGTCTC18380.1006197585892212No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18350.10045552612144772No Hit
GTTTATTACTGCATGCAAGGTTCATACTGGCCTCCGGGGCTCACTTTCGGCGGAGGGACCAAGTTGGAGATCACA18320.10029129365367424No Hit
GATGGGTCCTGTCCCAATTGCAGCTGCGGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGGAGACTCTGTCCCTCA18300.10018180534182525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)