FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910635
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22850.043.520781
GGTATCA7950.042.999011
TCAACGC26300.037.5244564
ATCAACG26900.036.8157543
TATCAAC26750.036.7642062
CAACGCA27050.036.6116035
AACGCAG27300.036.1499336
ACGCAGA29550.033.5104947
AGAGTAC30400.032.46180711
CGCAGAG30750.032.202778
CAGAGTA31350.031.69824210
CTATCCG450.00458436830.71821634
CGTATAC450.004617767430.672673
GCAGAGT33950.029.2690839
AGTACGG26000.029.1964213
GAGTACG26700.028.56020212
CGAATTA500.007726704727.60540215
GTGGTAT3600.026.8562831
TGGTATC3400.026.387522
GTACGGG29650.025.25313414
Produced by FastQC (version 0.11.5)