FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853996
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21650.041.5751151
GGTATCA8750.038.23091
TATCAAC25500.034.4563452
ATCAACG26000.033.926253
TCAACGC26250.033.603144
CAACGCA26750.033.3614735
GTGGTAT3750.033.1071741
AACGCAG27600.032.3340386
TACCGTA450.004652078330.626077
TAAGGCG1251.2551027E-1030.35179929
ACGCAGA29700.030.1620397
TGGTATC4000.030.1493032
AGAGTAC30250.029.38756211
CGCAGAG30850.029.0376838
CAGAGTA31200.028.49274810
GCAGAGT33350.026.7576379
GAGTACG25550.026.1625512
AGTACGG26050.025.79265813
ACTCTAT1300.001695608423.64450570
GTACGGG29000.022.93129214
Produced by FastQC (version 0.11.5)