FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853996
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10500.12295139555688786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10200.042.514431
GTATCAA26750.041.557051
TATCAAC30400.036.2129822
ATCAACG30550.036.035183
TCAACGC31050.035.4549034
CAACGCA31300.034.9518935
AACGCAG31650.034.5674066
ACGCAGA33200.032.861487
AGAGTAC34200.032.10564811
CGCAGAG34000.031.9889228
CAGAGTA34950.031.41668510
CCCGAAA702.9594186E-530.7329467
GCAGAGT36900.029.6631749
GAGTACG28800.028.32518212
AGTACGG28950.028.05952513
GTGGTAT4100.027.7010381
TGGTATC3850.027.7005842
TAAACGA500.00782140227.5363812
GTACGGT658.7786827E-426.4741861
AAGGCGC1608.0035534E-1125.82443223
Produced by FastQC (version 0.11.5)