FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1952006
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68170.34923048392269285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47800.040.9947171
GGTATCA19400.039.44781
TATCAAC53400.036.3696562
ATCAACG54050.035.7418253
TCAACGC54350.035.4814034
CAACGCA56350.034.2229585
AACGCAG57850.033.576296
ACGCAGA62450.031.0553077
GTGGTAT7550.030.9087621
CGCAGAG64650.029.9492558
AGAGTAC64800.029.82771511
CAGAGTA66500.029.11682710
GCAGAGT70400.027.5525869
TGGTATC8100.026.6882172
CGACCGG2907.4578566E-1125.97055870
AGTACGG52400.025.28963913
GAGTACG53900.024.71323212
GAGTACT27250.022.29940812
GTACTTT26850.022.12016514
TACGGGG28550.022.00551415
Produced by FastQC (version 0.11.5)