FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087153
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31000.045.0245361
GGTATCA10950.041.2299771
TCAACGC36700.037.7238664
ATCAACG36800.037.6213573
TATCAAC36900.037.522852
CAACGCA37200.037.4019855
AACGCAG38300.036.6874586
ACGCAGA40700.034.099417
CGCAGAG40750.033.974628
AGAGTAC41050.033.72633411
CAGAGTA41650.033.32316210
GCAGAGT43800.031.7660689
AGTACGG33750.030.20467213
GAGTACG34250.029.7637312
GTACGGG37800.026.96845814
TACGGGA11450.026.76829715
TACGGGG17600.025.04572115
TAACGGC2700.023.04016936
TTAACGG2700.023.03166635
ACGGGTA1653.2086973E-922.95857616
Produced by FastQC (version 0.11.5)