FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680953
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28390.41691570490180674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18200.26727248429774153No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16610.24392285517502676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16100.236433351494156No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14200.20853127895757856No Hit
GATTTTACTCTCACCATCAACGGCCTGCAGCCTGAAGATCTTGCAACATATTACTGTCAACAATTTGATGATGTC12530.18400682572806054No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12420.18239144258120604No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12380.18180403052780442No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12010.17637046903383935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11880.17446137986028404No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11240.16506278700585797No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11120.1633005508456531No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10880.15977607852524328No Hit
GAGTAAAATCTGTGCCAGATCCACTTCCACTGAACCTTGATGGGACCCCT10320.1515523097776205No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9970.1464124543103562No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG9880.14509077719020255No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9480.13921665665618627No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9370.1376012735093318No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9350.13730756748263095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9240.13569218433577648No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT9150.13437050721562283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9110.13378309516222117No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8940.13128659393526426No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8930.13113974092191386No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8820.12952435777505936No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8790.12908379873500814No Hit
ACATATTACTGTCAACAATTTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA8490.12467820833449593No Hit
GTCTGGGGGCTCGCACAATAGTAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTT8230.12086002998738532No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8140.11953835286723166No Hit
ATAGTAAACGGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCAACTGA7990.11733555766697554No Hit
GTAATATGTTGCAAGATCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTAAAATCTGTGCCAGATCCACTTCCA7950.11674814561357391No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA7790.1143984973999674No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7760.11395793835991617No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7540.1107271720662072No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7460.10955234795940395No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7450.10940549494605355No Hit
CTCTTACTCCAACCCGTCCCTCAGGGGCCGAGTCACCTTGTCAGTTGACA7350.10793696481254947No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7340.10779011179919906No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCTACGCCTGCGAAGTCA7300.10720269974579744No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG7190.10558731659894297No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7140.1048530515321909No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7070.10382508043873806No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT7000.10279710934528521No Hit
CTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6970.102356550305234No Hit
ATATTACTGTCAACAATTTGATGATGTCCCCCTCACTTTCGGCGGAGGGA6970.102356550305234No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6950.10206284427853318No Hit
CCCCTAAACTCCTGATCTACGATGCATCCACTTTGGAAACAGGGGTCCCA6840.10044746113167868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1900.046.930931
GTATCAA13850.044.5720061
TATCAAC15300.039.218452
TCAACGC16300.036.8124054
CAACGCA16600.036.1471255
AACGCAG16950.035.6056256
ATTGGAT750.002368040635.14686270
AGAGTAC17350.034.81542611
CAGAGTA17800.033.9352610
CGCAGAG17750.033.636728
GCAGAGT18200.033.189439
AACCCGA800.00324935132.9501870
CGCATTT450.004134610831.37211661
AACCACG450.004506641530.82462945
ATGTTAC450.00473544130.51418522
ATGCACG450.00473544130.51418524
ATACGAG450.004762749730.4783363
TAAGACC450.004762749730.4783364
AGTACGG17600.029.64076813
GAGTACG17950.029.06281512
Produced by FastQC (version 0.11.5)