FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248884
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTG11550.46407161569245114No Hit
CCTATGAGCTGACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACG10440.4194725253531766No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9000.36161424599411773No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC8800.3535783738609151No Hit
GCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACT8550.34353353369441186No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCT8200.3294707574613073No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCAC8040.3230420597547452No Hit
CCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCA7750.3113900451616014No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7390.29692547532183666No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6760.2716124781022484No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT6670.26799633564230724No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6590.2647819867890262No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6300.2531299721958824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG6260.2515227977692419No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT6160.24750486170264058No Hit
GGATAAGAATCGGCCCTCTGGGATCCCAGAGCGATTCTCTGGCTCCAATCTGGGGAATACGGCCACCCTGACCAT6080.24429051284935954No Hit
CTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTG6060.24348692563603927No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6030.2422815448160589No Hit
CAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC6010.2414779576027386No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG5870.2358528471094968No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT5810.23344208546953604No Hit
GGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGG5540.22259365808971246No Hit
GTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGGCCGTATTCCC5510.2213882772697321No Hit
GTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCT5460.21937930923643145No Hit
CCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGT5280.21214702431654905No Hit
GGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGC5180.20812908824994775No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC5080.20411115218334644No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA5030.2021021841500458No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4990.2004950097234053No Hit
GCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGG4980.20009321611674513No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG4950.19888783529676476No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGAC4920.19768245447678434No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG4680.1880394079169412No Hit
GTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCAGAGCG4650.18683402709696084No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA4630.18603043988364057No Hit
CTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT4590.18442326545700005No Hit
CCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGC4590.18442326545700005No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG4530.18201250381703926No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCAG4270.17156587004387586No Hit
CTCTCCTTCTGAGCCTCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCACTCTCAGTGT4250.1707622828305556No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC4180.1679497275839347No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC4110.16513717233731376No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA4050.162726410697353No Hit
GTAATAGTCAGCCTCATCCCCGGCTTGGGCTCTGCTGATGGTCAGGGTGG4010.16111923627071245No Hit
GGACAGCAGCGTGGCATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA3960.1591102682374118No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC3890.1562977129907909No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC3810.15308336413750986No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT3690.14826184085758828No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA3660.1470564600376079No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC3620.14544928561096734No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG3580.14384211118432685No Hit
GTATCAACGCAGAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCT3490.14022596872438564No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC3490.14022596872438564No Hit
CGGCCACCCTGACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTAT3450.13861879429774515No Hit
GCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCAAG3400.13660982626444448No Hit
CTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGGCGGAGGGACCA3370.1354044454444641No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG3370.1354044454444641No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTGCTGTCCC3330.13379727101782357No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACG3230.12977933495122226No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATGCCACGCTG3220.12937754134456214No Hit
CTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCCCCGGCTTGGGC3200.12857395413124187No Hit
GTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAA3180.1277703669179216No Hit
GAGTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAG3180.1277703669179216No Hit
CAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTACCTGTGGGGGATACAACATTGAAAGTAAAAATGTGCA3130.12576139888462096No Hit
GACCATCAGCAGAGCCCAAGCCGGGGATGAGGCTGACTATTACTGTCAGGTGTGGGACAGCAGCGTGGCATTCGG3090.12415422445798044No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG2950.11852911396473859No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC2920.1173237331447582No Hit
GACTCAGCCACTCTCAGTGTCAGTGGCCCTGGGACAGACGGCCACAATTA2870.11531476511145754No Hit
GGATACAACATTGAAAGTAAAAATGTGCACTGGTACCAGCAGAAGCCAGG2840.11410938429147716No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCCTCTGGGATCCCAGAGCGATT2830.11370759068481703No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG2810.11290400347149676No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC2780.11169862265151638No Hit
TCCTTGCTCACTTTACAGTTTCTGTGGCCTCCTATGAGCTGACTCAGCCA2760.11089503543819611No Hit
GTACGGGAGCCTGGGCCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGC2750.11049324183153597No Hit
AGCCAGGCCAGGCCCCTGTGCTGGTCATCAATAAGGATAAGAATCGGCCC2730.10968965461821573No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC2670.10727889297825492No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA2670.10727889297825492No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA2630.1056717185516144No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT2620.10526992494495427No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT2560.10285916330499349No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT2560.10285916330499349No Hit
CCTCAGGAAGCAGCATCGGGGGTGCCGCAGCCATGGCCTGGACCGCTCTCCTTCTGAGCCTCCTTGCTCACTTTA2500.10044840166503272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT501.6370905E-1161.9729271
TTGCACG200.006734814552.9104765
GTATTAC200.007093049652.2217853
AGATCGC200.00744159551.5922247
CGTATGC552.5247573E-950.3118741
TCGTATG552.5484042E-950.26109340
GGTATCA1900.047.113921
ACATGCG605.7134457E-945.87819327
CACGAGA605.7225407E-945.86897321
ACGAGAC605.7225407E-945.86897322
TGGTATC704.5292836E-1044.2219052
TGCCGTC803.274181E-1143.3594245
GTATGCC651.0986696E-842.60601842
CTTATAC651.1528755E-842.374651
TTATACA651.1630618E-842.3320772
TATACAC651.1630618E-842.3320773
ATGCCGT702.0942025E-839.60277644
CTCGTAT702.1740561E-839.4351439
CGAATTA350.001356715339.36372833
CGAGACA801.6752892E-938.70194223
Produced by FastQC (version 0.11.5)