FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548168
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGTCGTCATAGGA60960.3937557164338754No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC60340.38975098309744166No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGG54480.35189979382082565No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC51030.3296153905777668No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43710.28233370021858095No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41590.26864009590690413No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39110.25262116256116907No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38580.2491977614832499No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG38460.24842265180523046No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38380.24790591201988413No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35190.2273009130792007No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC34810.22484639909880583No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG34450.2225210700647475No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA34440.22245647759157922No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATTG33270.21489915823088968No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT32130.207535616289705No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31080.2007534066070349No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31010.2003012592948569No Hit
GTCCTATGACGACAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG30960.19997829692901545No Hit
ATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG30780.1988156324119863No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC30760.19868644746564973No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC30020.19390660445119653No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT29460.19028942595377246No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG29310.18932053885624817No Hit
GATTATTACTGCCAGTCCTATGACGACAGCCTGAGTGCCTGGGTGTTCGG27800.1795670754078369No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27710.1789857431493223No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27500.17762930121278828No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26750.17278486572516677No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA26500.17117005389595963No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25890.16722991303269413No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT25720.16613184098883324No Hit
CCCAGGCACTCAGGCTGTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGG25700.16600265604249667No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25630.16555050873031865No Hit
TCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGT25060.16186873775972632No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG24640.1591558538866583No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24300.15695970979893656No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC24160.15605541517458055No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAG23230.15004831516992986No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGC22910.1479813560285447No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22870.14772298613587156No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC22830.1474646162431984No Hit
CGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGG22470.1451392872091401No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG21980.14197425602389405No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21930.14165129365805262No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21680.14003648182884545No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT21600.13951974204349918No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21480.1387446323654797No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21460.13861544741914314No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC21430.13842166999963829No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC21100.13629011838508484No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20990.13557960118023368No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAT20810.1344169366632045No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG20480.13228538504865106No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACGACAGCCTGAGTGCCTGGGTGTTCGGCGGAGGGACCAAG20240.13073516569261218No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19950.12886198397073187No Hit
GCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT19680.12711798719518813No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG19580.12647206246350523No Hit
GCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAGCAGCTTCCA19400.12530939794647608No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19370.12511562052697123No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19220.12414673342944693No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG18890.12201518181489347No Hit
GCTTGGTCCCTCCGCCGAACACCCAGGCACTCAGGCTGTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCT18470.11930229794182544No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18390.11878555815647913No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC18090.11684778396143054No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17970.1160726742834111No Hit
GTGTACATCATAACGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGA17910.11568511944440138No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17890.1155559344980648No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17730.11452245492737223No Hit
GTGCCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC17720.11445786245420395No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17620.11381193772252106No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG17420.11252008825915535No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16990.10974261191291901No Hit
ATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGCTAACAGCAATCGGCCCT16740.10812780008371185No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT16720.10799861513737527No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG16670.10767565277153383No Hit
GGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTATGATGTACACTGGTACCAGCAGCTTC16640.10748187535202898No Hit
GATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACGTGCCCCGATGTTGGAG16620.10735269040569241No Hit
GTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTACATCATAACGT16540.10683595062034612No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA16530.10677135814717784No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16510.10664217320084125No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG16360.10567328610331696No Hit
GTCGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCC16120.10412306674727809No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16020.1034771420155952No Hit
CCCCTGAGGGCCGATTGCTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTACCAGTGTA15890.10263743986440749No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT15870.1025082549180709No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT15830.10224988502539777No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC15830.10224988502539777No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC15800.1020561076058929No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15670.10121640545470519No Hit
CTGTTAGCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTGCTGGTA15540.10037670330351744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)