FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842980
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCTGCGACGGTCACAGAGGTCAGGTTGAGGGAGA23530.27912880495385417No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22050.26157204204132956No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21090.25018387150347576No Hit
GTCTATTACTGTGCGAGACATCCCCCGTATTATGATCACCTTTGGGGGATTGAGCGTCCAGCAAGCATTGACTA19370.2297800659564877No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15930.18897245486251155No Hit
GTCTCTGGTGGCTCCATCAGTCCTTACTACTGGAGCTGGATCCGGCAGCC14790.17544900234881017No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14290.16951766352701134No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14230.16880590286839545No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAACTCCCAGATGGGTC13990.165958860233932No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13310.15789223943628555No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12030.1427080120524805No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11730.13914920875940118No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11680.1385560748772213No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11340.13452276447839806No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10630.1261002633514437No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10550.12515124913995587No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10360.1228973403876723No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10240.12147381907044058No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10110.11993167097677288No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10080.11957579064746494No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9880.11720325511874541No Hit
AGTCAGGGCATTGGCAGTTATTTAGCCTGGTATCAGCAGAAACCAGGGAA9870.11708462834230944No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG9820.11649149446012953No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT9820.11649149446012953No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9710.11518659991933379No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9550.11328857149635815No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9530.1130513179434862No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9290.11020427530902274No Hit
GTATAACAGTGCCCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9290.11020427530902274No Hit
CTCCCAGATGGGTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGCTGAAGCCTTCGGAGACC9160.10866212721535504No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA9140.10842487366248309No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9020.10700135234525136No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8990.10664547201594343No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA8710.10332392227573607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.040.3694841
GTGGTAT1900.039.933291
TATCAAC14850.034.832072
CACTGAT10200.034.67939470
TAACGCA400.00260194934.4837464
TCTATAC400.00260194934.4837463
ATCAACG15650.033.0515143
TCAACGC15800.032.7377364
CAACGCA16300.031.7335115
AACGCAG16600.031.577356
CGTCTAA450.00448533430.85537350
TGGATTG7150.029.31715870
TAACCGT605.1842746E-428.97015453
CAGAGTA17750.028.77312510
AGAGTAC17700.028.65944311
TTCGGGG9200.028.48066170
CGCAGAG18350.028.3947988
GCAGAGT18450.028.0555389
ATTATAC500.00775157227.5869963
TATACCA756.1813975E-527.5869965
Produced by FastQC (version 0.11.5)