FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270426
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25800.037.7316061
GGTATCA10850.034.9976431
GTGGTAT4900.034.5204051
TATCAAC28150.034.06572
ATCAACG29800.032.295273
CAACGCA30350.031.8236715
TCAACGC30300.031.7623464
AACGCAG30950.031.2067386
ACGATAG450.004496931630.84029445
TGGTATC5350.030.3036582
ACGCAGA33750.028.821017
CAGAGTA36000.027.11978110
CGCAGAG36300.026.895658
CCGTTAA906.917404E-626.82806816
AGAGTAC38000.025.51085311
GCAGAGT38650.025.4388589
AGTACGG33750.024.73681613
GAGTACG34700.024.05958412
TGTTAGA4900.022.65777470
TCGATCC953.066671E-421.78862812
Produced by FastQC (version 0.11.5)