FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539498
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57420.3729787242334839No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42680.2772332279743137No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40730.26456676137286306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36100.23449202272429062No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC35970.23364759161752727No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35660.23163394820909156No Hit
CTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA35160.22838613626000162No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT34750.2257229304617479No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34610.22481354311600274No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34220.22228024979571265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33570.2180580942618958No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA33240.21591453837549643No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC32120.20863943960953507No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31470.20441728407571816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29220.18980213030481363No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG28710.18648936211674194No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT28540.1853851060540514No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA28140.18278685649477946No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27180.17655105755252687No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATGGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA26430.171679339628892No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC25420.1651187594917304No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25380.16485893453580322No Hit
CTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACG24770.16089660395791355No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24690.16037695404605917No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC24320.15797357320373265No Hit
GGTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT24220.15732401081391464No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23960.1556351486003879No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCC23640.15355654895297038No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23110.1501138682869351No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG23050.1497241308530443No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23020.1495292621360989No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22480.1460216252310818No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21950.14257894456504652No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21740.1412148635464288No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA21610.1403704324396654No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21090.1369927080126119No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20720.13458932717028538No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGTTTTGG20450.13283550871777683No Hit
GTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTT20290.13179620889406807No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20210.1312765589822137No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCA20190.1311466465042501No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTGTCCCTGCCCGTCACCC20150.13088682154832287No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19220.12484589132301568No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGACTCAGTCTCCACTGTCC19220.12484589132301568No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGCTGA19210.12478093508403389No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18880.12263737919763457No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18800.12211772928578017No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18800.12211772928578017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18670.1212732981790168No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18540.1204288670722534No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18470.11997417339938082No Hit
GTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT18230.11841522366381768No Hit
TTATTACTGCATGCAAGCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA18210.11828531118585409No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18110.11763574879603612No Hit
AGTCAGAGCCTCCTGCATAGTTTTGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG17900.11627166777741836No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17850.11594688658250936No Hit
GTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCC17780.11549219290963678No Hit
GTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA17600.11432298060796442No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAACC17590.11425802436898262No Hit
GTCTCCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT17490.11360846197916463No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17430.11321872454527385No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17220.11185464352665608No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17210.11178968728767429No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17070.1108802999419291No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCCCTGA17020.11055551874702013No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17010.11049056250803833No Hit
CCACTGTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG16980.11029569379109294No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16860.10951621892331136No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16780.10899656901145699No Hit
GAGGTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT16610.10789231294876642No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16440.10678805688607584No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCAAAACTATGC16330.10607353825727607No Hit
CTCCACAGCTCCTGATCCATTTGGGTTCTACTCGGGCCTCCGGGGTCCCT16330.10607353825727607No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16020.10405989484884033No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15950.10360520117596775No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15870.10308555126411338No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15830.10282572630818618No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAACCTCCGTGGACGTTCGGCCAAGGGA15830.10282572630818618No Hit
GTTTATTACTGCATGCAAGCTCTACAACCTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA15690.101916338962441No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAACCT15500.10068217042178684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.046.3698921
GTGGTAT2950.044.4278031
TGGTATC3000.042.5017472
GTATCAA23200.040.585221
ATACGGA350.001355539.38385816
CAGTTAG1101.1621318E-535.23277770
TCAACGC27400.034.460884
TATCAAC27500.034.4608762
ATCAACG27550.034.3983353
CAACGCA27850.033.9040575
AACGCAG28600.033.135466
TTTAGCG450.004445682330.91303651
CGCAGAG30950.030.6175338
AGAGTAC30900.030.3335111
TGTTAGA10050.030.2078770
CAGAGTA31350.030.00801810
TTATACC756.212673E-527.5686994
TAAGGTA500.00777867127.5686994
TAATACG500.00777990427.56780414
ACGCAGA34800.027.2302477
Produced by FastQC (version 0.11.5)