FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590865
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCACAATCTC58140.365461557077439No Hit
GATTATTACTGTGCAGCATGGGATGACAGGCTGGATGGTGTGGTATTCGG57900.3639529438387292No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51220.32196320869464096No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCT43380.2726818428967888No Hit
GTGTGGTATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC42920.2697903341892618No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG39970.251246963130121No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCACACCATCCAGCCTGTCATCCCATG39440.24791544222797032No Hit
GTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGCAGAGGGTCAC39420.24778972445807784No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37320.23458935861936747No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37000.23257787430108778No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG34620.21761745968388266No Hit
ATACCACACCATCCAGCCTGTCATCCCATGCTGCACAGTAATAATCAGCC32970.20724574366775306No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32290.20297133949140878No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30420.1912167280064619No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAACCTGCAGCTCTAGTCTCC30310.19052528027205326No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCACACCATCCAGC30030.18876523149355853No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30020.18870237260861228No Hit
CATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28060.17638203115914927No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG27610.17355338133656847No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTC27530.17305051025699855No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26940.1693418360451704No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25970.16324452420538513No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAACCTGCAG25000.15714721236559984No Hit
CCCCTGAGGACAGTAGATCGTCAGAATAGATGAGGCGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCAGTTGA24810.15595289355162129No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24280.15262137264947057No Hit
CTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCTGTCAACTGGTACCAGCAGCTCCCAGGAAAGGCTCC23950.15054702944624465No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23600.14834696847312626No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG23540.14796981516344881No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23460.1474669440838789No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22660.1424382332881797No Hit
CATCTATTCTGACGATCTACTGTCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC22300.14017531343011508No Hit
CCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGT21990.13822668799678164No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20850.13106077511291028No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAACCTGCAG20270.12741495978602838No Hit
TCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAG20060.12609492320215732No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19850.12477488661828627No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19840.12471202773334004No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19620.12332913226452277No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAA19600.12320341449463026No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGGCTGG19340.12156908348602805No Hit
GGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGT19330.1215062246010818No Hit
CTACTGTCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGG18700.11754611484946868No Hit
GAATAGATGAGGCGTTTGGGAGCCTTTCCTGGGAGCTGCTGGTACCAGTTGACAGCATTATTTCCGATGTTGGAG18670.11735753819462998No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18530.1164775138053826No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18460.11603750161075892No Hit
GGGTCACAATCTCCTGTTCTGGAAGCAGCTCCAACATCGGAAATAATGCT18090.11371172286774806No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18060.11352314621290932No Hit
GCATGGGATGACAGGCTGGATGGTGTGGTATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCCAAGG17890.11245454516882324No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGTCTGAAGCCCCCAGGC17650.1109459319301135No Hit
GTCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG17500.11000304865591988No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGT17490.10994018977097365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17390.10931160092151125No Hit
GTGTATTACTGTGCGAAAGACCGTGGGTACGGTGATTACGGTAGTGGCTACTTCTACTCTATGGACGTCTGGGGC17360.10912302426667253No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGC16950.10654580998387671No Hit
GTAATACACAGCCGTGTCGTCAGCTCTCAGGCTGTTCATTTGCAGATACA16480.10359144239140343No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16420.10321428908172597No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCGGTGT16290.10239712357742486No Hit
CTCCAGCATGGCCTGGTCCCCTCTCTTCCTCACCCTCATCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG16290.10239712357742486No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCACACCATCCAGCC16090.10113994587850005No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16030.10076279256882262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)