FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409090
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTT350.007050435552.30039270
GTATCAA14150.043.5556031
GGTATCA5400.040.8069041
TATCAAC16250.037.8990632
ATCAACG16450.037.4382863
TCAACGC16600.037.0999874
CAACGCA17100.036.0151945
AACGCAG17550.035.0917246
ACGCAGA18850.032.854137
AGAGTAC19450.031.84063511
CGCAGAG19500.031.5825548
CAGAGTA19950.030.87016510
GCAGAGT20350.030.4324479
GAGTACG13900.030.19769912
AGTACGG13900.030.19769913
TACGGGG6250.028.62543715
GTACGGG14550.028.61219614
TACGGGA4550.027.22199215
GAGTACT8050.024.78910812
AGTACTT8350.023.89848313
Produced by FastQC (version 0.11.5)