FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409090
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4520.1104891344202987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG300.00336877763.02773370
TATGTCG300.00336877763.02773370
GTATCAA14950.043.605721
ACGGGTA405.1648407E-542.8832816
TCAACGC16950.037.8486064
TATCAAC17000.037.737292
ATCAACG17050.037.626623
CAACGCA17500.036.8551185
GGTATCA5400.036.2169761
AACGCAG18100.035.822946
ACGCAGA18700.034.673547
CGCAGAG18900.034.3108188
CAGAGTA19300.033.2441610
AGAGTAC19500.032.90319411
GCAGAGT20300.031.9445559
AGTACGG13300.031.47308213
GAGTACG13350.031.35520712
CGAACTA553.3287937E-431.241329
CGGTGTT450.00474233530.50223421
TACGGGG5450.029.5855315
Produced by FastQC (version 0.11.5)