FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138163
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAAC451.7058119E-646.54240451
GTCCCGA306.416171E-445.8180711
ATTACTC551.5033402E-743.735433
CGTTGAA602.7559508E-740.48644642
GAGTAAG350.001367703439.2868541
AATAGCG556.3944954E-638.40489262
AATCGTT557.2003586E-637.74708639
TAATCGT557.2003586E-637.74708638
GACTTTA557.400962E-637.5963733
CCGGTCT557.5498283E-637.487519
TCCGGTC557.5498283E-637.487518
GTATCAA5150.036.7122271
TAATAGC601.1731938E-535.1523561
ACCTTTA601.2063565E-535.0097756
CGAACCT601.215538E-534.9710953
ACGAACC708.781626E-734.9196452
TCGTTGA601.2879265E-534.6773241
ATCGTTG601.2943909E-534.65200840
GTAGGAC601.35719165E-534.41337629
ACTCCGG601.370738E-534.3635526
Produced by FastQC (version 0.11.5)