FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373234
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62190.45287256214163063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.040.7754861
GGTATCA14250.037.83421
ATCAACG37850.035.645113
TCAACGC38300.035.1366654
CAACGCA39100.034.5055585
TATCAAC39350.034.286342
AACGCAG41150.032.9570246
CGCAGAG44600.030.430938
ACGCAGA44550.030.3030977
CGAATTA1052.431807E-829.44390515
AGAGTAC46550.029.22997711
CAGAGTA49350.027.71078910
GCAGAGT51250.026.6164179
GAGTACG35350.025.46624212
TTACACG951.0920159E-525.2957574
AACGTTC700.001251496324.914249
TACGGGT5150.024.6795215
AGTACGG36700.024.52947213
GTGGTAT6050.024.4069141
CCGTTAA2001.8189894E-1224.04147516
Produced by FastQC (version 0.11.5)