FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624911
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22690.3630917042586864No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT21050.33684796715052223No Hit
GTAATATACGGCCGTGTCCGCGGCAGTAAGAGACCTCAGTTTCAGGGAGA19060.3050034324887864No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17940.2870808803173572No Hit
GTATAAAAACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17800.28484056129592855No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15990.255876436804601No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14750.2360336111862329No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14250.22803247182398775No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT14190.22707233510051833No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGA13990.22387187935562022No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13910.22259169705766102No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13410.2145905576954158No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12610.2017887347158235No Hit
GTCATAGATGTACCCAATCGACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCTCCAGTAGTAACCACCA12450.19922837011990507No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12220.19554784601327227No Hit
GAGTGAACTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCA12090.19346754977908853No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC12040.19266743584286403No Hit
GGATACCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG11570.18514636484235356No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC11480.1837061597571494No Hit
CTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT11180.17890547613980232No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC11040.17666515711837366No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT10970.17554499760765932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10960.17538497482041443No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAAAAACTGG10740.17186447350102654No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT10740.17186447350102654No Hit
GTCTCAGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG10530.16850399496888357No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10510.16818394939439377No Hit
GTTTTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT10370.1659436303729651No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10280.164503425287761No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10260.16418337971327118No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10000.16002278724490368No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9560.1529817846061279No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9490.1518616250954136No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9480.15170160230816868No Hit
GTATATTACTGTGCGAGAGGGGGTTCAGGCATGCTGATGGTGTATGGCCCCTTTGACTACTGGGGCCAGGGAAAC9200.1472209642653114No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9190.14706094147806648No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9150.14642085032908686No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9120.14594078196735216No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9080.14530069081837255No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC8680.1388997793285764No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8640.13825968817959677No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC8520.13633941473265793No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATA8350.13361902734949457No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8280.13249886783878023No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8120.1299385032428618No Hit
ATCTATGACAGTGGGAGCACCTACTACAATCCGTCCCTCAAGAGTCGAGT8050.12881834373214746No Hit
CCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGCC8050.12881834373214746No Hit
GTCTCCCGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG8040.12865832094490257No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT8030.12849829815765765No Hit
CATCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCA7910.1265780247107188No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7880.1260979563489841No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACTCTGTCCCAGACCCACTGGCA7800.12481777405102487No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7610.12177734109337168No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTA7450.11921697649745323No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG7400.11841686256122873No Hit
GTAGTAGGTGCTCCCACTGTCATAGATGTACCCAATCGACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCT7300.11681663468877969No Hit
CACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGC7230.11569647517806535No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7150.11441629288010613No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7130.11409624730561632No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6970.11153588270969787No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6880.11009567762449372No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAACAGTGATGACGCAGTC6800.10881549532653449No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC6550.1048149256454119No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6500.10401481170918739No Hit
GTGTATGGCCCCTTTGACTACTGGGGCCAGGGAAACCTGGTCACCGTCTC6430.10289465219847306No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC6420.10273462941122816No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6410.10257460662398325No Hit
GAACTGCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC6350.10161446990051383No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAGAGACAGGGTGGCGGG6350.10161446990051383No Hit
GTGCCAGTGGGTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTA6340.10145444711326893No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6260.10017426481530971No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG150.002359217368.96918527
TCGTATG200.00730769351.83891740
ATTGCGC200.007379809451.710338
GGTATCA3050.044.0950551
GTATCAA7500.042.30111
TACACAT451.00557125E-438.3039475
TACTGAT8050.037.35237570
CTTATAC400.00260112334.4845921
CTATGGA400.002605209634.4735572
CATACTG501.8688508E-434.4735575
TATCAAC9350.033.9205062
ATCAACG9950.031.8750463
TCAACGC9950.031.8750464
CAACGCA10200.031.0937965
AACGCAG10200.031.0937966
TATGGAT450.00463845930.643163
TTCGGGG6050.029.82015870
TGTTAGA3750.028.50954470
AGAGTAC10900.028.46668211
GAGCAAA8800.028.09445870
Produced by FastQC (version 0.11.5)