FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624911
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22660.3626116358969517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19130.30612359199950073No Hit
GTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT18590.29748236148827595No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16280.2605170976347032No Hit
GTATAAAAACTGGCCTCGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT16110.25779671025153983No Hit
GTAATATACGGCCGTGTCCGCGGCAGTAAGAGACCTCAGTTTCAGGGAGA15010.24019420365460042No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13580.21731094507857918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13370.2139504665464362No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13280.2125102614612321No Hit
GTCTGGGACAGAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATT12760.20418907652449708No Hit
CATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGA12500.2000284840561296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12320.1971480738857213No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC11990.19186732190663952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11510.18418622811888413No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT11230.17970559007602682No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTC11070.17714522548010836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10990.17586504318214913No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10970.17554499760765932No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10930.17490490645867973No Hit
GAGTGAACTCTGTCCCAGACCCACTGGCACTGAACCTGGCTGGGATACCA10780.17250456465000616No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC10430.16690376709643454No Hit
CTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT10050.16082290118112819No Hit
GGATACCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG10010.16018281003214857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10000.16002278724490368No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9870.15794249101071994No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9850.15762244543623014No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC9770.1563422631382709No Hit
TCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCACT9670.15474203526582184No Hit
GTTTTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACT9500.15202164788265848No Hit
GTCATAGATGTACCCAATCGACTCCAGGCCCTTCCCTGGGTGCTGGCGGATCCAGCTCCAGTAGTAACCACCA9430.15090148837194417No Hit
GTCTCAGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG9370.14994135164847475No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT9290.14866116935051552No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9280.1485011465632706No Hit
GAGTTCACTCTCAGCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAAAAACTGG9230.1477010326270461No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAACAGTGATGACGCAGTC8750.14001993883929073No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8640.13825968817959677No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8620.13793964260510697No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8570.13713952866888243No Hit
GTATATTACTGTGCGAGAGGGGGTTCAGGCATGCTGATGGTGTATGGCCCCTTTGACTACTGGGGCCAGGGAAAC8480.1356993235836783No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC8420.1347391868602089No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8400.13441914128571908No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAGCAACTTAGCCTGGTA8110.12977848045561688No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7970.12753816143418822No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC7940.1270580930724535No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7790.12465775126377997No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7780.12449772847653506No Hit
GTCTCCCGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTATCAG7780.12449772847653506No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT7730.12369761454031054No Hit
CATCTATGATGCGTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGCCAGTGGGTCTGGGACAGAGTTCA7600.12161731830612678No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGATA7590.12145729551888187No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7580.12129727273163697No Hit
CCAGTGGCCCTGGTGGACGCATCATAGATGAGGAGCCTGGGAGCCTGGCC7470.11953702207194304No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGCTGAGAGTGAACTCTGTCCCAGACCCACTGGCA7450.11921697649745323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7430.11889693092296343No Hit
ATCTATGACAGTGGGAGCACCTACTACAATCCGTCCCTCAAGAGTCGAGT7220.11553645239082044No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7190.11505638402908573No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7050.11281606500765709No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6930.11089579156071826No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT6820.1091355409010243No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6610.10577506236888133No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6590.10545501679439154No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAGAGACAGGGTGGCGGG6550.1048149256454119No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGATAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC6550.1048149256454119No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6450.10321469777296287No Hit
GTCCCAGGTGCACCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACGCTGTCCCTCACCTGCAATGT6430.10289465219847306No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6340.10145444711326893No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6270.10033428760255461No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA6260.10017426481530971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)